Human apolipoprotein A-I gene promoter mutation influences plasma low density lipoprotein cholesterol response to dietary fat saturation》.pdfVIP

Atherosclerosis 137 (1998) 367–376 Human apolipoprotein A-I gene promoter mutation influences plasma low density lipoprotein cholesterol response to dietary fat saturation Pedro Mata a, Jose Lopez-Miranda b, Miguel Pocovi c, Rodrigo Alonso a, Carlos Lahoz a, Carmen Marin b, Carmen Garces a, Ana Cenarro c, Francisco Perez-Jimenez b, Manuel de Oya a, Jose M. Ordovas d,* a Fundacion Jimenez Diaz, Madrid, Spain b Hospital Uniersitario Reina Sofia, Cordoba, Spain c Facultad de Ciencias, Uniersidad de Zaragoza, Zaragoza, Spain d Jean Mayer USDA, Human Nutrition Research Center on Aging at Tufts Uniersity, Boston, MA, USA Received 12 December 1996; received in revised form 29 October 1997; accepted 30 October 1997 Abstract Previous studies have shown that the A to G transition occurring at position 75 bp upstream of the transcriptional start site in the human apolipoprotein A-I gene may affect plasma high density lipoprotein cholesterol (HDL-C) levels and low density lipoprotein cholesterol (LDL-C) response to changes in amount of dietary fat. We have examined the response to dietary fat saturation as a function of this mutation in 50 men and women. Subjects were first fed a saturated (SAT) fat diet (35% fat, 17% SAT) for 28 days, followed by a diet rich in monounsaturated fatty (MUFA) acids (35% fat, 22% MUFA) for 35 days and a diet rich in polyunsaturated (PUFA) fat (35% fat, 13% PUFA) for 35 days. All meals were prepared and consumed at the study sites. Lipoproteins were measured at the end of each diet period. The allele frequency for the A allele was 0.13. Subjec