Effects of dietary fat amount and saturation on the regulation of hepatic mRNA and plasma apolipoprotein A-I in rats》.pdfVIP

Atherosclerosis 152 (2000) 69–78 locateatherosclerosis Effects of dietary fat amount and saturation on the regulation of hepatic mRNA and plasma apolipoprotein A-I in rats Lucı´a Calleja a, Marı´a Carmen Trallero a, Carmen Carrizosa b, Marı´a Teresa Me´ndez b, Evangelina Palacios-Alaiz b, Jesu´s Osada a,* a Departamento de Bioquı´mica y Biologı´a Molecular y Celular, Facultad de Veterinaria, Uniersidad de Zaragoza, Miguel Seret, 177, E -50013 Zaragoza, Spain b Instituto de Bioquı´mica, ( Centro mixto CSIC- UCM) , Facultad de Farmacia, Ciudad Uniersitaria, Madrid, Spain Received 22 December 1998; received in revised form 27 September 1999; accepted 3 November 1999 Abstract The effects of the amount of dietary fat and saturation together with cholesterol both on hepatic apolipoprotein A-I gene mRNA levels and on plasma levels of this apolipoprotein were studied in male rats. To achieve these goals, seven groups of male Wistar rats were established: control group (n 5) consuming chow diet; cholesterol group (n 4) fed on a chow diet containing 0.1% (ww) cholesterol; coco group (n 5) fed on a chow diet containing 0.1% (ww) cholesterol and 40% coconut oil; corn group (n 5) fed on a chow diet containing 0.1% (ww) cholesterol and 40% corn oil; and three olive groups consuming a chow diet containing 0.1% (ww) cholesterol and percentages of 5 (n 5), 10 (n 4) and 40% (n 5), respectively, of olive oil. Animals were kept on these diets for 2 months and then sacrificed for lipoprotein, apolipoprotein and hepatic mRNA analysis. Dietary cholesterol