《暗室环境可抑制氨己烯酸的视网膜毒性》.pdfVIP

中国临床神经科学2009 年第 17 卷第5 期 Chin J Clin Neurosci 2009, 17 (5) ,457~462 457 论 著 暗室环境可抑制氨己烯酸的视网膜毒性 张 瑜, 王庆平, 叶 纹 (复旦大学附属华山医院眼科200040) 关键词 氨己烯酸；光照；视网膜毒性 摘要 目的：观察新型抗癫 药氨己烯酸的视网膜毒性与光毒性的关系，探索氨己烯酸视网膜损伤的预防机制。方 法：选用30 只健康雄性BALB/c 小鼠。正常治疗组(n= 10) ，置于正常饲养环境中(黑暗与光照交替12 h) ，给予氨己烯 酸250 mg ·kg-1 ·d-1 腹腔注射；暗室治疗组(n= 10) ，24 h 置于暗室环境中，药物治疗浓度同正常治疗组；对照组(n= 10) ，置 于正常饲养环境中，给予0.9% NaCl 0.1 mL ·d-1 ，腹腔注射。1个月后处死小鼠取视网膜进行5 种抗体(Anti-VGLUT 、Anti- Bsaaoon 、Anti-Go 、Anti-PKC 和Anti-calbindin ) 的免疫荧光染色。结果：5 种抗体染色表明暗室治疗组小鼠的视杆 细胞突触、ON - 双极细胞突触、水平细胞突触由视网膜外丛状层(OPL) 向外核层(ONL) 的退缩在暗室环境中可被抑制。 结论：氨己烯酸的小鼠视网膜毒性有光依赖性，去除光毒性的影响，小鼠视网膜第一与第二级神经元之间的病理性突 触改变可被完全抑制。 Neuronal Plasticity Induced by Vigabatrin Can Be Suppressed by Darkness in the Mouse Retina ZHAN G Yu, WAN G Qing-Ping, YE Wen Department of Ophthalmology, Huashan Hospital, Fudan University, Shanghai 200040, China KEY WORDS vigabatrin; retina; toxicity ABSTRACT Aim: To detect the correlation between phototoxicity and vigabation-elicited retinal damage, and explore the potential management of vigabatrin toxicity on retina. Methods: 30 BALB/c male mice were separated to three groups. Ten mice received vigabatrin daily intraperitoneal inj ection were kept in normal day-night cycle situation as vigabatrin treatment group; another ten mice were kept in darkness during the whole process of vigabatrin intraperitoneal inj ection as vigabatrin darkness group; the residual ten animals which accepted vehicle solution inj ection were also kept in normal day-night cycle situation as the control group. The whole period of experiment also last thirty days, vigabatrin concentration and dose of inj ection were the same among them. The animals were sacrificed after 30 days and the retinas were taken out. Their immunostaining reactions with the five kinds o