应用原子力显微镜分析苯甲酸钠生物毒性.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2008, August 25; 24(8): 1428-1432 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究报告 应用原子力显微镜分析苯甲酸钠生物毒性 1 2 1 1 1 胡明铅 , 王炯坤 , 蔡继业 , 吴扬哲 , 王小平 1 暨南大学化学系, 广州 510632 2 暨南大学组织移植与免疫实验中心, 广州 510632 摘 要: 应用原子力显微镜(Atomic force microscope, AFM)在单细胞水平上研究食品防腐剂苯甲酸钠(Sodium benzoate, SB)的生物毒性, 从可视化的角度分析了淋巴细胞与不同浓度 SB 作用不同时间后其形态及其膜超微结构的影响。结果 与正常淋巴细胞相比, 随着与淋巴细胞作用的 SB 浓度和作用时间的增加, 细胞形态及细胞膜明显发生改变, 其超微结 构也趋复杂。经 SB 作用后的细胞高低差Rp-v 、均方根粗糙度Rq 、平均粗糙度Ra 、平均高度Z 4 个几何参数值均明显 发生改变。对经 SB 作用后的淋巴细胞进行统计学分析, 并探讨了其作用机制。 关键词: 原子力显微镜, 苯甲酸钠, 淋巴细胞, 形态结构 Analysis of Sodium Benzoate Biotoxicity by Atomic Force Microscope 1 2 1 1 1 Mingqian Hu , Jiongkun Wang , Jiye Cai , Yangzhe Wu , and Xiaoping Wang 1 Chemistry Department, Jinan University , Guangzhou 510632, China 2 Institute of Tissue Transplantation and Immunology, Jinan University , Guangzhou 510632, China Abstract: Atomic force microscope (AFM) was used to study biotoxicity of food preservative sodium benzoate (SB) at the single cellular level. Lymphocyte morphology and membrane ultrastructure treated with SB at different concentrations and time were analyzed visually. As compared to the normal lymphocyte, the cell morphology and membrane was significantly changed and its ultrastructure was also complicated. After treated with SB, the Rp-v, Rq, Ra and Z values were changed. The statistical analysis of lymphocytes after treated wit