黑暗诱导烟叶衰老过程中质体色素代谢规律研究_赵二卫.pdfVIP

2010, 22( 5): 1~ 4 Acta Agriculturae Jiangx i 赵二卫, 赵宸楠, 姚珊珊, 崔红 (, 450002) : 以烟草栽培品种 K326为实验材料, 在中部叶叶龄 50 d时, 对其进行 独叶片黑暗诱导衰老( ind ividuall darkened leaves, IDLs)处理, 研究了烟叶质体色素及其降解成分含量变化叶片衰老和质体色素代谢的关键基因表达规律结果表明: 在衰老过程中叶绿素和类胡萝卜素都在不断降解, 在 IDLs处理的第5 d时成熟特征显著; 在 IDLs第5 d衰老基因表达明显增 强或减弱, 色素合成基因表达减弱, 分解基因表达显著增强 : 黑暗诱导; 烟叶衰老; 质体色素; 基因表达; 代谢规律 : S572. 01 : A : 1001- 8581( 2010) 05- 0001- 04 Study onM etabolism Regularity of Plastid Pigm ent in Individually Darkened Senescent Tobacco Leaves ZHAO Er- we,i ZHAO Chen- nan, YAO Shan- shan, CUIH ong (College of T obacco Science, H enan Agricu lturalUniversit ; Ke Laborator ofT obacco Industrial Cultivation, Zhengzhou 450002, China) Abstract: The tobacco cu ltivarK326 w as used as expermi entalm aterial in this stud , when them iddle leaf of tobacco was 50 da s old, its senescencew as rapidl induced b treatm ent of darkened induced senescence ( DIS) to individual leaf, the changeab le regular- it of ch loroph ll and carotenoid content, the am ount of plastid pigm ent catabolites neutral arom a constituent, the expression of the genes related to leaf senescence and plastid pigm ent w ere studied. The resu lts showed that both ch loroph ll and carotenoid w ere de- graded constan tl in the course of leaf senescence. On the fifth da afterDIS treatm ent, them ature characteristic of leafw as evident, the expression of the senescent genes was increased or decreased, the genes of p lastid pigm ent bios nthes is were w eakl expressed, wh ile the genes of plastid pigm ent degradation were evidentl strongl expressed. Key words: IDLs; T obacco leaf senescence; Plastid pigm ent; Gene expression; M etabolism regularit [ 7, 8] , , , [ 1] [ 9