水稻ILP标记遗传图谱的构建.pdfVIP

HEREDITAS (Beijing) 2008 2 , 30(2): 225―230 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00225 ILP 1, 2 1 赵向前 , 吴为人 1. , 310029; 2. , 314016 内含子长度多态性(ILP)是一种基于 PCR 的新型分子标记, 具有许多突出的优点。我们先前利用已公布 的籼稻品种93-11 和粳稻品种日本晴的基因组序列数据, 已开发了172 个水稻ILP 标记。为了检验这些ILP 标 记的可靠性及其在遗传作图中的可用性, 利用一个BC F ( 日本晴/93-11// 日本晴)群体, 构建了一张含172 个ILP 1 1 标记座位和 13 个SSR 标记座位的水稻遗传图谱, 总长度为1 905.7 cM。比较显示, 图谱上所有标记的顺序与其 物理顺序完全一致, 证明了利用ILP 标记进行遗传作图的可行性和有效性。文中还对标记偏分离现象进行了分 析, 发现在第6 号染色体短臂上存在一个严重偏分离的区域。 水稻; ILP 标记; 遗传图谱 Construction of a genetic map based on ILP markers in rice ZHAO Xiang-Qian1,2, WU Wei-Ren1 1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; 2. Jiaxing Academy of Agricultural Science, Jiaxing 314016, China Abstract: Intron length polymorphism (ILP) is a new type of PCR-based molecular markers with many advantages. We had previously developed 172 ILP markers in rice using the published genome sequence data of indica cultivar 93-11 and ja- ponica cultivar Nipponbare. In order to examine the reliability and the applicability of these ILP markers to genetic map- ping, we constructed a rice genetic map consisting of 172 ILP and 13 SSR markers with a total length of 1 905.7 cM using a BC F (Nipponbare/93-11//Nipponbare) population. Comparison showed that the order of the markers in this map was ex- 1 1 actly the same as their physical order, verifying the feasibility and efficiency of using ILP markers for genetic mapping. We also investigated the phenomenon of marker segregation distortion and found a region of serious segregation distortion in the short arm of chromosome 6. Keywords: rice; ILP marker; genetic linkage map