英文硫辛酸和乙酰左旋肉碱联合用药对帕金森病细胞模型的预防作用.pdfVIP

J. Cell. Mol. Med. Vol 14, No 1-2, 2010 pp. 215-225 Combined R-–lipoic acid and acetyl-L-carnitine exerts efficient preventative effects in a cellular model of Parkinson’s disease Hongyu Zhang a, # , Haiqun Jia a, b , Jianghai Liu a , Ni Ao a , Bing Yan a, b , Weili Shen a , Xuemin Wang c , Xin Li a , Cheng Luo a , Jiankang Liu d, e, * a Institute for Nutritional Science, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, China b Graduate School of the Chinese Academy of Sciences, Beijing, China c Department of Biochemistry, Second Military Medical University, Shanghai, China d Institute for Brain Aging and Dementia, University of California, Irvine, CA, USA e Institute of Mitochondrial Biology and Medicine, Xi’an Jiaotong University School of Life Sciences and Technology, Xi’an, China Received: November 8, 2007; Accepted: May 10, 2008 Abstract Mitochondrial dysfunction and oxidative damage are highly involved in the pathogenesis of Parkinson’s disease (PD). Some mitochondrial antioxidants/nutrients that can improve mitochondrial function and/or attenuate oxidative damage have been implicated in PD therapy. However, few studies have evaluated the preventative effects of a combination of mitochondrial antioxidants/nutrients against PD, and even fewer have sought to optimize the doses of the combined agents. The present study examined the preventative effects of two mitochondrial antioxidant/nutrients, R-–lipoic acid (LA) and acetyl-L-carnitine (ALC), in a chronic rotenone-induced cellular model of PD. We demon- strated that 4-week pretreatment with LA and/or ALC effect