二氮嗪停搏液对缺血再灌注心肌线粒体的保护作用.docVIP

二氮嗪停搏液对缺血再灌注心肌线粒体的保护作用 作者:李寒,张载高,范士志 作者单位：海军总医院心外科,北京 100037;第三军医大学大坪医院野战外科研究所心胸外科 ,重庆 400042 【摘要】 目的 观察线粒体三磷酸腺苷(ATP)敏感钾离子通道开放剂二氮嗪加入停搏液内对缺血再灌注大鼠心肌线粒体保护作用。方法 50只大鼠随机分为5组，每组10只，取离体心脏，A 组取正常心肌，其余四组置于Langendorff模型。B组停灌30 min, 复灌60 min；其余各组分别灌注不同的停搏液：C组灌注停搏液；D组灌注含二氮嗪的停搏液；E组灌注含二氮嗪停搏液之前10 min给予格列苯脲。各组复灌60 min取标本，电镜下观察线粒体结构改变；提取线粒体，激光共聚焦显微镜扫描检测线粒体膜电位变化；测定线粒体呼吸功能；高效液相法测定心肌ATP含量及能荷(EC)变化。结果 缺血再灌注心肌线粒体结构损伤和功能降低(P0.05)；而二氮嗪处理组相应指标显著改善(P0.01)。结论 含二氮嗪停搏液能够改善缺血再灌注心肌线粒体结构和功能。 【关键词】 心肌缺血再灌注损伤;线粒体;线粒体呼吸控制率;二氮嗪 　　Protective Effect of Cardioplegia with Diazoxide to Myocardial Mitochondrion during Ischemic and Reperfusion in Isolated Working Rat Heart 　　LI Han,ZHANG Zai-gao,FAN Shi-zhi,CHEN Jian-ming, JAING Yao-guang 　　(Department of cardiothoracic Surgery of Navy General Hospital,Beijing 100037;Department of cardiothoracic Surgery Daping Hospital of the Third Military Medical University,Chongqing 400042,China) 　　Abstract: OBJECTIVE To evaluate the effects of mitochondrial ATP- sensitive potassium channel opener diazoxide on heart mitochondrion during ischemic and reperfusion in rats. METHODS Fifty rats were divided into 5 groups randomly. Langendorff model was established. Control group (B) underwent 30 minute ischemia and 60minute reperfusion. Group C and D were treated with St.Thomas Ⅱ cardioplegia and diazoxide+St.Thomas Ⅱ cardioplegia before ischemia respectively. And group E was treated with glibenclamide at 10min before Diazoxide+st.Thomas Ⅱ cardioplegia. At the end, mitochondrial changes were detected under the transmission electron microscope(TEM),the respiration function of mitochondrion was determined,ATP,ADP,AMP content of myocardium were determined and energy charge (EC) were calculated. RESULTS Ischemic and reperfusion injury induced the structure damaged and function decrease of myocardial mitochondrial. While in group D(P0.01), the heart was protected effectively by treating with diazoxide. CONCLUSION These results sugges