不同培养基对铁皮石斛组培快繁的影响.pdfVIP

南方农业学报 2011．42(4)：349—352 JournalofSouthernAgnculture ·349· Effectofdifferentculturemediaonmicr0pr0pagatiI)n0f Dendrobium candidum W al1．exLindl HUANGChang-yan，LIKui-peng，YANGMei-chun ，ZHOU Shan (AgriculturalCollege，GuangxiUniversity，Nanning530005，China) Abstract：【Objective】Theexperimentswereconductedtofindoutthesuitableculturemediumfortheseedsgermi— nation，budmultiplication，rootingand growth ofplantletinDendrobium candidum Wal1．exLindl，andtodevelop atech— niqueforitsrapidpropagation．【Method】SeedsofDendrobiumcandidumwereusedasexplantsintissueculture，andthe effectsofdifferentmediawithdifferentconcentrationsof6-BA (0—3．0mlg／L)andNAA(0．1-0．3mg／L)，supplementedwith mashedbanana(15-25％)ormashedpotato(15—25％)，ontheseedgermination，budmuhiplication，rootingandgrowthof plantletswereinvestigated．R【esult]ThemostsuitableculturemediumforseedgerminationandbudmultiplicationwasMS+ 20％mashedpotatoandMS+6-BA (2．0mg／L)，respectively．However，thebestsuitablemedium forrootingwasfoundMS+ 15％mashedbananawhichgave100％rootingrate．【Conclusion】Thestrongrootingplantlctcanbeobtainedinmedium supplementedwithmashedpotatoandmashedbanana．Thismedium mayberecommendedofrthelargescaleproductionof Dendrobium eandidum plantlets． Keywords：Dendrobium candidum Wal1．exLindl；tissueculture；seedgemr ination；budmuhiplicztion；rooting CLC number：$567．9 Docmu entcode：A Article：2095-1191(2()11)04—0349—04 不同培养基对铁皮石斛组培快繁的影响 黄 昌艳 ，李魁鹏 ，杨美纯 ，周 珊 (广西大学农学院，南宁 530005) 摘要：【目的】探讨铁皮石斛种子萌发、芽增殖和生根的适宜培养基 ，为铁皮石斛组织快繁技术提供依据。【方法】 以铁皮石斛种子为外植体进行培养，研究6一BA(0—3．0rag／L)、NAA(0．1—03mg／L)、香蕉泥05％～25％)和马铃薯泥 (15％～ 25％)对铁皮石斛种子萌发、芽增殖和生根壮苗的影响。【结果 】适宜种子萌发