采用CZE的研究人趋化素样因子1+C端活性多肽和肝素的相互作用.pdfVIP

采用CZE研究人趋化素样因子lC端活性多肽与肝素的相互作用 刘一1，张殊屿2，凌笑梅2，王英3，李玉珍111北京大学人民医院药剞礼北京100044；2．北京大学药学院药物分析教研室． 北京100191：3北京大学人类戎病基因中心．北京100191) CZE)研究人趋化素样因子l 摘要：目的采用毛细管区带电泳法l C端活性多肤CKLFl．C19／C27与肝素的相互作用，并进 一步研究CKLFI·C19与肝素相互作用的关键氧基酸残基结合位点．方法在pH7．2的Tris．醋酸缓冲溶液中， CKLFl-C 肽链上的赖氨酸残基在相互作用的过程中发挥了重要作用，脯氨酸残基也会影响CKLFI．C19和肝素问的结合．结论本 CKLFI-C19和CKLFI-C27在体内的活性及其生物学意义打下了基础。 关键词：毛细管区带电泳；CKLFl；肝素；肽；相互作用 CZEto interactionsbetweenthe fromsecretedhumanCKLFIand Using stud),the peptides heparin LIUY11，ZHANG Xiao．mei2，WANG Shu．yu2，LING Yin93，L1Yu．zhenl(1．脚栅洲ofPh口册哪Peking j i ofPharmaceutical Sciences UniversiO’PeopleHospital．Beoing00044．China：2．DepartmentAnalysis,SchoolofPharmaceutical Human Disease 100191．China) PekingUniversio．Bering100191．China：3．CenterforGenomics，PekingUniversiO；BeUing ABSTRACT：OBJECTI％’Easelective methodbasedon Using high-performanceanal)qical capillaD’zone to imeractionsbetween and CZE to structural investigate heparinCKLF卜C27／CKLFl-C19．Meanwhile,Usingstud)the of andCl也Fl·C19 requirementsheparin recognition．METHODSSamplescontaining and atvariousratioswere at bv incubatedroom andthen CZEwith C19km／CKLFl-C19pmheparin temperatureseparated was Tris·acetatebu舵rat 7．2．Eachrun within6rain．RESIfLTSBoth and characterizations pH completed qualitativequantitative ofthe weredetermined．TheconstantsoftheimeractionsbetweenCKLF卜C27／CKLFI-C19and were binding