不同培养容器等因素对杉木组培苗生根及影响.pdfVIP

不同培养容器等因素对杉木组培苗生根的影响 马志慧1 丁国昌1 林思祖1，2 1.福建农林大学，林学院，福州,350002 2.福建省杉木工程技术研究中心，福州，350002 摘要：本文探讨了培养容器的类型、培养基种类、活性炭及培养时间对杉木无性系FS21的生根率、平 均根长、平均根条数的影响。结果表明：杉木无性系FS21宜在以培养基1为基本培养基、未添加活性炭 的培养基上进行生根培养，且透气性良好的组培瓶对该无性系的生根影响较大。添加活性炭能降低杉木 组培苗的生根率和平均根条数，但能增加平均根长，在生根培养30d后，二号和四号培养基中FS21的平 均根长分别比未添加活性炭的分别增加2.35倍和16.22％,在生根培养35d后，二号和四号培养基中 FS21的平均根长分别比未添加活性炭的分别增加2.14倍和1.79倍。 关键词：杉木;培养容器;组织培养;生根率 EffectsofDifferentCultureVesselsonRoot FormationofChineseFirSeedlingsInVitro MAZhi-hui1DINGGuo-chang1LINSi-zu1,2* 1.College-ofFore,y,FujianAgricultureandForestryUniversity,Fuzhou350002, 2.FujianResearchCenterofChineseFirEngineering,Fuzhou350002) Abstract:Thispaperdiscussestheeffectsoftherootingrate,averagerootlengthandtheaveragenumberofroots oftheChinesefircloneFS21whichwerecausedbythetypesofculturevesselandculturemedium,activatedchar coalandculturetime.TheresultsshowthattheChinesefircloneFS21issuitableforrootingculturewhenmedium 1isusedasthebasicmedium,inwhichwithoutactivatedcharcoal,andthegoodpermeabilityofthecultivation bottleshaveagreatinfluenceontherootingofthisclone.Addingactivatedcharcoalcanreducetherootingrateand theaveragenumberofrootsofChinesefirseedlings,butcanincreasetheaveragerootlength.After30daysoftoo tingculture,theaveragerootlengthofFS21whichculturedinmedium2and4respectivelyincreased2.35times and16.22％ comparedwiththenumberswhenwithoutactivatedcharcoaLAfter35daysofrootingculture,theav eragerootlengthofFS21whichculturedinmedium2and4respectivelyincreased2.14timesand1.79timescom paredwiththenumbersobtainedwithoutaddingactivatedcharcoal. Keywords:Cunninghamialanceolata;culturevessel;tissueculture;rootingrate 巴性系粥2l j母疆U，j蠹点 b％o杉木无1 2为基本培赛 『根条数要fl ：添加活性爿 ：炭时，由刁 ·胙田蹄由碰 __【r7H ；性荔 幔率。 i荣，耿广习 艺，(05)：2