PBSPLA共混材料的制备和其细胞相容性研究.pdfVIP

2010;30(7) 南方医科大学学报(JSouthMedUniv) 窑1501窑 PBS/PLA共混材料的制备及其细胞相容性研究 1 2 2 1 1 2 1 华 琨 袁王小威 袁张 维 袁周 建 袁杨秀滨 袁季君晖 ( 北京协和医学院中国医学科学院阜外心血管病医院袁 2 北京100037曰 中国科学院理化技术研究所袁北京100190) 摘要院目的 本论文对聚丁二酸丁二醇酯/ 聚乳酸渊PBS/PLA冤合金作为胸骨固定材料的细胞相容性和细胞毒性进行了 深入地研究遥 方法首先采用CCK-8试剂盒表征PBS/PLA合金浸提液对L929 细胞生长增殖的影响曰其次将细胞直接 种于PBS/PLA 材料的表面评价该合金与L929 细胞的相容性情况曰最后利用PE袁PVC 作为对照材料袁评价合金对细胞 的毒性级别遥结果和结论 PBS/PLA合金对于L929 细胞具有良好的细胞相容性且无明显细胞毒性袁在其表面的繁殖塑 料甚至优于医用聚乙烯材料遥 证明PBS/PLA合金能够作为胸骨捆扎固定材料遥 关键 院聚丁二酸丁二醇曰聚乳酸曰胸骨固定曰细胞毒性曰细胞相容性 中图分类号院R654 文献标识码院A 文章编号院1673-4254渊2010冤07-1501-04 CytocompatibilityofPBS/PLAblendasthesternalfixationmaterial 1 2 2 1 1 2 HUAKun ,WANGXiao-wei,ZHANGWei,ZHOUJian,YANGXiu-bin,JIJun-hui 1DepartmentofSurgery,CardiovascularInstituteandFuwaiHospital,ChineseAcademyofMedicalSciencesandPekingUnionMedical 2 College,Beijing100037,China; InstituteofPhysicsandChemistry,ChineseAcademyofSciences,Beijing100190,China Abstract:ToevaluatebiocompatibilityandcytotoxicityofPBS/PLAblendasthematerialforsternalfixation. MethodsL929 cell line was used to assess the cytotoxicity and cytocompatibility of PBS/PLA blend by incubation of thecellswith the aqueousextractofthematerialfor3days. Thecytocompatibilityofthematerialwasevaluatedbyobservingtheproliferation ofthecellsculturedonthesurfaceofthematerial. Theproliferationofthecellsculturedonthesurfaceofthematerialwas compared with that of the cells cultured on medical polyethylene and polyvinyl chloride to assess the cytotoxcity of the material. Results L929 cellsshowedno morphological changes aftera3-dayincubationwiththe aqueousextract ofthe material. Thecellsculturedonthesurfaceoftheblendmaterialexhibitedbetterproliferativeactivitythanthoseculturedon medicalpolyethy