HBV前S1抗原和HBV_DNA_HBV_M的关系.pdfVIP

·20 · 分子诊断与治疗杂志 2009 年5 月第1卷第1期 J Mol Diagn Ther May 2009, Vol. 1 No. 1 ·论著 · HBV前S1抗原与HBV-DNA、HBV-M的关系 欧阳文婷 曾智杰 孙艳虹 [摘 要] 目的 探讨乙型肝炎病毒前S1抗原（HBV PreS 1-Ag）与HBV-DNA 和HBV 表面标志物 （HBV-M ）以及转氨酶之间的关系。 方法 从临床检测HBV-M 的标本中筛出HBV 阳性病例150例，采用 ELISA 法检测乙肝前S1抗原和荧光定量PCR 法检测标本HBV-DNA ，并与25 例HBsAb(+)和5 例HBV-M 全阴 性血清进行比较，同时采用酶法检测所有血清标本的天冬氨酸氨基转移酶（AST ）和丙氨酸氨基转移酶 （ALT ）。 结果 HBV-DNA 和HBV PreS 1-Ag 在HbeAg(+)组中检出率分别为100%和90.1% ，在HbsAg(+) ＋ HbeAb(+) ＋HbcAb(+) 组中检出率为48.1% 和40% 。在HbsAg(+) ＋HbcAb(+) 和单纯HbsAg(+) 两组中， HBV-DNA 与前S1 抗原仍有一定检出率。HBV PreS 1-Ag(+)组中，ALT 与AST 异常率分别占54.3% 和 46.7% 。 结论 HBV-DNA 检测为反映乙肝病毒复制最灵敏、特异的方法，乙肝前S1抗原检测成本低廉， 与HBV-DNA 的符合度较高，是对乙肝“两对半”和HBV-DNA 测定的重要补充和加强，适合在没有条件开展 HBV-DNA 定量的广大基层医院推广。 [关键词 HBV-M ；HBV-DNA ；前S1抗原] The relationship of HBV PreS 1-Ag and HBV-DNA、HBV-M in patients with hepatitis B virus infection OUYANG Wenting, ZENG Zhijie, SUN Yanhong (Department of Laboratory Science, The 1st affiliated hospital of Sun Yat-sen University, Guangdong, Guangzhou 510080, China) [ABSTRACT] Objective To study the relationship between HBV PreS 1-Ag、HBV-DNA 、HBV-M and hepatic enzyme in patient infected with HBV. Methods The serum pre-S 1 antigen and HBV-DNA were tested by ELISA and real-time fluorescent quantitative PCR(RT-PCR) in 150 patients infected with HBV screening HBV-M by ELISA method. At the mean time, 25 HBV-Ab(+) and 5 HBV-M negative samples were tested. Aspartate aminotransferase(AST) and alanine aminotransferase(ALT) were tested by rate method in all samples. Results The positive rates of the pre-S 1 antigen and HBV-DNA were highest in HbeAg ’s positive patients with different serology models of hepatitis B virus infection, which were 90.1% and 100% separately. The positive detection rates for pre-S 1 antigen and HBV-DNA in