抗肺癌相关蛋白ERGIC3单克隆抗体制备和应用.pdfVIP

抗肺癌相关蛋白 ERGIC3 单克隆抗体的制备和应用 1 1 2 1 1* 林清海 ，张葵东 ，魏万里 ，段何仙 ，曹 毅 (1．中国科学院昆明动物研究所，云南昆明 650223；2．云南省肿瘤医院，云南昆明 650118) 摘要：肺癌是一种常见肿瘤，其死亡率居恶性肿瘤之首，其患者的5 年生存率为10%到15%。本实验室在前期的 工作中通过SSH（抑制性消减杂交技术）建立肺腺癌的差异表达基因文库，筛选出在肺癌细胞中高表达的ERGIC3 基因。本研究通过制备ERGIC3蛋白的单克隆抗体，探究ERGIC3蛋白在人体正常组织以及常见肿瘤组织中的表达 情况，为研究ERGIC3在人体中的生理及病理功能的提供依据。 关键词：肺癌；ERGIC3；单克隆抗体 Development of A New Monoclonal Antibody Toward Lung Cancer-related Protein ERGIC3 1 1 2 1 1* LIN Qinghai , ZHANG Kuidong , WEI Wanli , DUAN Hexian , CAO Yi (1. Key Laboratory of Animal Models and Human Disease Mechanism, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China; 2. Yunnan Tumor Hospital, Kunming 650118, China) Abstract: Lung cancer is a common cancer, the mortality rate ranks first in cancer, the 5-year survival rate of its patients is 10% to 15%. In previous study, we constructed cDNA libraries of differentially expressed genes using lung adenocarcinoma by suppression subtractive hybridization, and subsequently found over-expression of ERGIC3 in lung cancer. In this study, we developed a new monoclonal antibody (mAb) toward ERGIC3 protein. The mAb binds to natural ERGIC3 in lung cancer cells with the high specificity and the high affinity. Furthermore, we used the mAb to investigate the ERGIC3 expression in human normal tissues and common tumor tissues. The new mAb is useful for the study of ERGIC3 physiological and pathological functions, and may also be applied