鸡沙门氏菌.pdfVIP

Clinical Immunology (2008) 126, 89–96 ava i l a b l e a t w w w. s c i e n c e d i r e c t . c o m w w w. e l s ev i e r. c o m / l o c a t e / yc l i m Cloning, expression and characterization of heat shock protein 60 (groEL) of Salmonella enterica serovar Typhi and its role in protective immunity against lethal Salmonella infection in mice Piyush Kumar Paliwal, Anju Bansal, Sarada S.K. Sagi, Sairam Mustoori ⁎, Ilavazhagan Govindaswamy Genetic Engineering Group, Experimental Biology Division, Defence Institute of Physiology and Allied Sciences (DIPAS), Lucknow Road, Timarpur, Delhi-110054, India Received 20 February 2007; accepted with revision 21 September 2007 Available online 19 November 2007 KEYWORDS Abstract Heat shock proteins (Hsps) represent dominant antigens in numerous microbial groEL; infections, suggesting a potential use of pathogen-derived Hsps for vaccination. The present Salmonella; study evaluates the immunogenicity and protective efficacy of groEL (Hsp60) of Salmonella Immunity; enterica serovar Typhi against lethal challenge by S. Typhi Ty2 and Salmonella enterica serovar Heat shock proteins Typhimurium in mice. The groEL gene was cloned and expressed in Escherichia coli BL21 and purified by affinity chromatography. Immunization of mice with groEL resulted in a significant increase in antibody titers. Antibody isotyping revealed that groEL immunization induces both IgG1 and IgG2a antibodies. There was a significant increase in