苏丹红与牛血清白蛋白作用的光谱学研究interactionofbovineserumalbuminandsudan.pdfVIP

Food and Chemical Toxicology 49 (2011) 3158–3164 Contents lists available at SciVerse ScienceDirect Food and Chemical Toxicology journal homepage: www.elsevi /locate/foodchemtox Binding of Sudan II and Sudan IV to bovine serum albumin: Comparison studies Dawei Lu 1, Xingchen Zhao 1, Yingcan Zhao, Bingcong Zhang, Bin Zhang, Mengyang Geng, Rutao Liu ⇑ Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, China–America CRC for Environment Health, Shandong Province, 27# Shanda South Road, Jinan 250100, PR China a r t i c l e i n f o a b s t r a c t Article history: In this paper, we report the interaction of Sudan II and Sudan IV to bovine serum albumin (BSA). Struc- Received 17 July 2011 tural analysis showed that both Sudan II and Sudan IV interact mainly with BSA at the hydrophobic Accepted 12 September 2011 pocket and via Van der Waals forces. The number of bound Sudan molecule for each protein molecule Available online 17 September 2011 was approximately 1. The overall binding constants at 293 K (20 C) estimated for Sudan II and Sudan IV were 1.22 104 M1 and 1.48 104 M1, respectively. BSA backbone structure was damaged by the Keywords: dyes with more severe phenomenon observed for Sudan IV. For two Sudan dyes with the same concen- Sudan II tration, Sudan IV could cause more alterations on CD spectra o