水稻与白叶枯病菌互作的基因表达谱分析与差异性表达基因的识别.pdfVIP

中国农业科学 2007,40 2 :277-282 Scientia Agricultura Sinica 水稻与白叶枯病菌互作的基因表达谱分析与差异性 表达基因的识别 吴茂森，田 峰，齐放军，何晨阳 （中国农业科学院植物保护研究所／植物病虫害生物学国家重点实验室，北京 100094 ） 摘要：【目的】从基因组水平上阐明水稻在与白叶枯病菌 Xanthomonas oryzae pv. oryzae ，Xoo 感病互作 条件下基因表达反应的特征。【方法】用 cDNA-AFLP 方法对 Xoo 接种处理不同时间的水稻细胞进行基因表达谱分 析。【结果】在测定的大约4 000个水稻cDNA片段中，363个 9.1% 是差异性表达的，其中295个受Xoo诱导上 调表达，68 个被抑制下调表达。根据在不同互作时间点的表达特征，它们可分为 7 种表达类型。对 31 个差异片 段的序列同源性进行分析，发现它们在己公布的水稻全基因组中完全相同的序列，但只有10个基因具有已知或推 断的生物学功能，其余21个基因功能未知。用荧光实时定量PCR分析法证实了cDNA-AFLP基因表达结果。【结论】 构建了水稻－Xoo感病组合在细胞水平上互作的基因表达谱，从基因组水平上识别了一批受Xoo诱导或抑制表达、 与水稻感病反应相关的基因，这些新基因的发现为开展水稻感病功能基因组学的研究提供了材料。 关键词：水稻；白叶枯病菌；cDNA-AFLP；表达谱；差异性表达基因 cDNA-AFLP Analysis of Gene Expression Response to Xanthomonas oryzae pv. oryzae and Identification of Genes Expressed Differentially During this Compatible Interaction in Rice Suspension Cultured Cells WU Mao-sen, TIAN Feng, QI Fang-jun, HE Chen-yang （State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094 ） Abstract: 【Objective 】The study was conducted to elucidate the gene expression response of rice to bacterial inoculation with Xanthomonas oryzae pv. oryzae Xoo at the genomic levels. 【Method 】cDNA-amplified fragment length polymorphism cDNA-AFLP was employed to analyze the gene expression patterns of mock- and Xoo-inoculated rice suspension cultured cells. 【Result 】Three hundred and sixty-three 9.1% from approximately 4 000 cDNA fragments analyzed were differentially regulated 295 up-regulated and 68 down-regulated 0.5, 3, 24 h after co-cultivation with Xoo. Seven gene expression patterns of the differential fragments were identified. Ten of 31 sequenced fragments showed homology to genes with known or putative functions involved in metabolism, pathogen response and signaling. 21 others did not show any homology to sequences with known functions. cDNA-AFLP differential patterns for 5 sele