马钱子总生物碱中其他生物碱对马钱子碱体外透皮吸收性质的影响.pdfVIP

Chinese Journal of New Drugs 2010, 19( 17) ! ! 1, 2, 3 1 1, 2, 3 , , ( 1南京中医药大学, 南京 210029; 2 江苏省中药炮制重点实验室, 南京 210029; 3 国家教育部中药炮制规范化及标准化工程研 中心, 南京 210029) [] : 考察马钱子总生物碱中马钱子碱透皮吸收性质: 测定总生物碱中马钱子碱的油 水分配系数; 用改良的 Franz扩散池法, 选择离体大鼠腹部皮肤为渗透屏障, 高效液相色谱(HPLC) 法测定其 中马钱子碱的浓度: 与马钱子碱对照品相比, 总生物碱中马钱子碱在 pH 5, 7. , 9的磷酸盐缓冲液 ( PBS) 中的油水分配系数均无显著性差异; 在 pH 7. 和 9的 PBS 中, 透皮吸收速率分别提高 1. 0和 1. 26 倍(P 0. 05), 累积透过率也有显著提高(P 0. 05), 而在 pH 5的 PBS 中透皮吸收性质无显著性差异 : 总生物碱中其他生物碱在游离型状态时对马钱子碱的透皮吸收有一定的促进作用 [] 马钱子总生物碱; 马钱子碱; 油水分配系数; 透皮吸收 [] R [] A [] 1003- 373 ( 2010) 17- 1517- 0 Transdermal absorption characteristics of brucine in vitro is affected by other alkaloids in totalalkaloids from seed ofStry chnos nux vom ica 1, 2, 3 1 1, 2, 3 LILei , CHEN Jun , CA IBaochang ( 1N anj ing University of ChineseM ed ic ine, N anj ing 210029, Ch ina; 2 J iangsu K ey Laboratory of ChineseM edicine P rocessing, N anj ing 210029, Ch ina; 3E ng ineering Center of StateM inistry of Educationf or Standardi ation of ChineseM edic ine P rocessing, N anj ing 210029, Ch ina) [Abstract] Objective: To study the transdermal absorption characteristics ofbrucine and effects of other al kaloids from seed of S trychnos nux vom ica in v itro. Methods: The oilw ater partition coefficient of brucine w as measured. Drug perm eation testsw ere performed through isolated rat sk in in an mi proved Franz diffusion cel.l Bru cine concentration in samplesw as determ ined by HPLC. Results: Compared to brucine reference substance, no significant d ifference w ere found in the oilw ater par