白斑综合征病毒引起小龙虾疫病爆发流行的病原学的研究.pdfVIP

参考文献 [1] Fulton, R.J., McDade, R.L., Smith, P.L, et al. Advanced multiplexed analysis with the FlowMetrix system [J]. Clin Chem. 1997, 43:1749-1756. [2] Dunbar, S.A., Vander Zee, C.A.. Oliver, K.G., et al. Quantitative, multiplexed detection of bacterial pathogens: DNA and protein applications of the Luminex LabMAP system [J]. J Microbiol Methods. 2003, 53:245-252 [3] Iannone, M.A., Taylor, J.D., Chen, J., et al. Multiplexed single nucleotide polymorphism genotyping by oligonucleotide ligation and flow cytometry [J]. Cytometry. 2000, 39:131-140. [4] JIANG Han-liang, ZHU Hai-hong, ZHOU Lin-fu, et al. Genotyping of human papillomavirus in cervical lesions by L1 consensus PCR and the Luminex xMAP system [J]. J Med Microbiol, 2006, 55: 715-720. 白斑综合征病毒引起小龙虾疫病爆发流行的病原学研究 1 1 2 王忠发 何伟贤 许文军 1 舟山市疾病预防控制中心水产养殖病害防治院（316000） 2 浙江海洋水产研究所（316100） 【摘要】 目的：为了了解本次养殖小龙虾疫病爆发流行的病原体，为沿海地区养殖小龙虾疫病 控制提供科学依据。方法：采用流行病学调查分析，对发病小龙虾进行病原学检测与组织病理变化 观察、病原体回归健康小龙虾。结果：在发病小龙虾各组织内均能检测到 WSSV ，组织病理变化明 显，小龙虾体内WSSV 的形态与PCR 扩增产物与对虾体内WSSV 一致，病原体回归与流行病学调 查支持本次检测结果。结论：养殖小龙虾本次疫病爆发流行的病原体是WSSV 。 【关键词】 白斑综合征病毒（WSSV ）；小龙虾；疫情爆发 The Investigation on the epidemic outbreak of disease of Crawfish caused by WSSV 1 1 2 Wang Zhong-fa , He Wei-xian , Xu Wen-jun 1 Zhoushan Center for Disease Control and Prevention, Zhoushan, 316000 2 Zhejiang Provincial Institute of Marine Products,Zhoushan,31600,China 【Abstract 】 Objective: To find out the pathogen of cultural crawfish which caused an epidemic outbreak of disease in Zhoushan and provide scientific base for disease control in littoral. Methods: The epidemiological inv