壳聚糖_羧甲基纤维素膜的制备及预防肠粘连的实验研究.pdfVIP

2006 Jul; 27( 7) 755 Acad J Sec Mil Med Univ 论 著 - * 肖海军, 侯春林 , 官士兵, 刘亚平 ( , 200003) [ ] 目的: 制备壳聚糖-羧甲基纤维素膜并探讨其预防肠粘连的效果方法: 将壳聚糖和羧甲基纤维素按1 1 质量比混 , 加入戊二醛硫酸铝铵双交联, 甘油增塑后烘干成膜; 测定膜的拉伸强度及断裂伸长率并行电镜扫描4 只SD 大鼠随机 分成为3 组(n = 16) , 2 组擦伤其回肠浆膜, 制成肠粘连模型, 于损伤部位分别包裹壳聚糖-羧甲基纤维素膜壳聚糖膜; 另 一组 不作处理作为对照组术后第14 天观察各组肠粘连情况结果: 膜拉伸强度约为20 MPa, 断裂伸长率约为 65% ; 扫描电镜 ( SEM) 显示膜表面结构呈相互交错的纤维状, 表面有不规则的孔状结构壳聚糖-羧甲基纤维素膜组和壳聚糖膜组的粘连程 度显著低于对照组(P 0.01) 光镜下见壳聚糖-羧甲基纤维素膜壳聚糖膜组炎症反应轻微, 纤维增生不显著; 对照组粘连处 纤维组织增生活跃, 局部炎症反应明显透射电镜下见壳聚糖- 羧甲基纤维素膜组上皮细胞修复较壳聚糖膜组好, 成纤维细胞 增生不明显, 对照组上皮细胞增生较慢, 成纤维细胞分泌胶原功能活跃结论: 壳聚糖-羧甲基纤维素膜适于术中缝 需要, 预防肠粘连效果显著, 性能优于壳聚糖膜 [ ] 壳聚糖; 羧甲基纤维素; 肠; 粘连 [ ] R 619 [ ] A [ ] 025 - 79X(2006) 07-0755-05 Preparation and evaluation of chitosan-carboxymethylcellulose membrane for prevention of postoperative intes- tinal adhesion:an experimental study * XIAO H a-i jun, H OU Chun-lin , GUAN Sh-i bing, LIU Ya-ping( Depart ment of Orthopedics, Changzheng Hospital, Second Military Medical University , Shanghai 200003, China ) [ABSTRACT] bjective:To prepare and evaluate the effect iveness of chitosan-carboxymethylcellulose membrane in preventing post operative intest inal adhesion. Methods: Chitosan- carboxymethylcellulose membrane was prepared w ith 1 1 ratio of chi- tosan and carboxymethylcellulose. Glutaraldehyde and ammonium aluminium sulfate w ere used for cross-linkage, glycerin for enhancing plasticit y; and then the product was dried. The membrane was observed w ith scanning electron microscopy ( SEM ) and it s tensile strength and breaking elongation were measured. Forty- eight SD rats w ith ileum injury w ere randomly divided in- to A, B and C groups ( n= 16) . During operat ion, the injury in group A was treated w ith chitosan-carboxymethylcellulose mem- brane, in group B with chitosan memb