血管内皮生长因子在高原脑水肿形成中作用的实验研究.pdfVIP

( ) ·362 · Chin J Clin Neurosci 2005 ,13 4 ,362～364 血管内皮生长因子在高原脑水肿形成中作用的实验研究 邓志宽 　钱桂生 　黄桂君 　赵世福 (第三军医大学附属新桥医院神经内科 , 呼吸内科研究所 400037) 关键词 　血管内皮细胞生长因子 ; 　高原脑水肿 ; 　血脑屏障 ( ) 摘要 　目的:探讨血管内皮生长因子 VEGF 在高原脑水肿形成中的作用 。方法 :建立大鼠模拟高原模型 ,应用脑干湿重比率法定量 脑水肿情况 、应用荧光素钠透过率测定BBB 通透性 、应用实时荧光定量 RTPCR 法检测脑组织 VEGF mRNA 含量以及应用蛋白印迹 法半定量脑组织 VEGF 含量 。结果 :大鼠在高原 24 h 后脑组织含水率明显增高 ( P 005) ,荧光素钠透过率显著增加 ( P 001) ; VEGF mRNA 转录及其表达显著增高 ( P 0001) 。结论 :VEGF 表达在高原脑水肿形成中起重要作用 。 An Experimental Study on the Role of Vascular Endothelial Growth Factor in the Formation of High Altitude Cerebral Edema 　D EN G ZhiKuan , QIAN GuiS heng , HUAN G Gui J un , ZHA O S hiFu 　Department of Neurology , Institute of Respiratory Disease ,the Second Affiliated Hospital ,the Third Military Medical University Chongqing ,400037 , China KEY WORDS 　vascular endothelial growth factor ; 　high altitude cerebral edema ; 　blood brain barrier ABSTRACT　Aim : To investigate the role of vascular endothelial growth factor in the formation of high altitude cerebral edemaMethods :Wistar rats were exposed to lowpressure cabin for 24 hours and mimiced high altitude of 7 000 meters The ratio of dry and wet brain weight was calculated and the sodium fluorescein leakage was calculated to evaluate the cerebral edema and the blood brain barrier permeability Also the brain tissue was processed for Western blots to detect VEGF expression and for realtime quantitative RTPCR to detect expression of VEGF mRNA Results :After 24 hours of rats exposed in mimiced the high altitude of 7 000 meters ,The ratio of water content of brain tissue increased significantly ( P 005) ,th