褐飞虱共生菌抗吡虫啉菌株和敏感菌株解毒酶活性的比较.pdfVIP

浙江农业学报Acta AgriculturaeZhejiangensis22(5)：653—659，2010 褐飞虱共生菌抗吡虫啉菌株和敏感菌株解毒酶活性的比较 李 娜1，陈建明2…，张珏锋2，何月平2，陈列忠2 (1杭州师范大学生命与环境科学学院．浙江杭州310036；2浙江省农业科学院植物保护与微生物研究所，浙江杭州310021) 摘要：比较测定了褐飞虱共生菌抗吡虫啉菌株和敏感菌株3种解毒酶活性的变化。结果表明，褐飞虱共生 菌不同抗感吡虫啉菌株3种解毒酶活性差异显著(P0．05)，其中抗吡虫啉菌株的羧酸酯酶、多功能氧化酶 活性显著高于敏感菌株，而谷胱廿肽一s一转移酶活性差异不显著。说明褐飞虱共生菌对吡虫啉抗药性的提高， 与其体内羧酸酯酶、多功能氧化酶的活性增强有关。 关键词：褐飞虱共生菌；抗吡虫啉菌株；敏感荫株；解毒酶活性；抗药性 中图分类号：S482．3+9 文献标识码：A foractivitiesof inresistant-and Comparison detoxifyingenzymes susceptible-imi- strainsofricebrown daclopridendosymbiotic planthopper，Nilaparvatalugens S话I U Nal，CHENJian．min92一，ZHANGJue．fen92，HEYue—pin92，CHENLie．zhon92 (1 Environment Normal Collegeof嘶and Sciences，HangzhouUnwe瑙Sy，Hangzhou310036，China；2Instituteof PlantPro抛ctionand 310021，China) Microbiology，ZhejiangAcademyofAgriculturalSciences，Hangzhou of inresistant··and strainsofrice Abstract：Activities detoxifyingenzymes susceptible··imidaclopridendusymbiotic brown were resultsshowedthatresistant—and planthopper，Nilaparvatalugens compared．The susceptible-imidaclo- strainshad differenceinactivitiesof of enzymes，inwhich，activities prid significant detoxifying earboxylesterase， mixedfunctionoxidasein strainswere thanthosein resistant··imidaclopridsignificantlyhigher susceptible·-imidaclo- of showednoobviousdifferencebetweenresistant-and strains，while prid activityglutathione-S-transferase suscepti— strains．Itwas thatresi