反相液相色谱移动相组成对蒽系列单磺酸在ODS柱上保留行为的影响.pdfVIP

反相液相色谱移动相组成对蒽系列单磺酸在ODS柱上保留行为的影响.pdf

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反相液相色谱移动相组成对蒽系列单磺酸在ODS柱上保留行为的影响.pdf

7. B.N,Ames,J.Mccann and E.Yamasaki, were extracted with CH2Cl2.Extracts were Mutat.Res,31,347(1975). filtered, concentrated, and prefractionated on silica 8. D.B.Clayson and R.C.Garner,“Chemical gel cartridges with hexane. CH2Cl2.and CH30H, Carcinogens.ACS Monograph 173”,American The CH2Cl2 fraction was diluted to the desired Chemical Society, Washington. DC 366 concentration with the HPLC mobile phase (35% (1976). n-propanol in 0.05M monocbloroacetic acid- 9. M.L.Yu and R,A,Hites.Anal.Chem.53. sodium acetate buffer at pH 3.8) and 20μ was 951(1981) injected into the HPLC system consisting of a l0. P,T,Kissinger,K.Bratin,W.P.King and pump. 25 cm 4mm i.d.ODS column main- J.R.Rice, ACS Symp.Ser.,136.57(1981). tained at 50 in a column oven,and an electro- 11. S. M.Rappaport.Y.Y.Wang.E.T.Wei, chemical detector with a glassy carbon working R,Sawyer.B,E,Watkins and H.Rapoport, electrode operated at potential of -0.6V vs.a Environ, Sci, Technol.,14,1505(1980). Ag-AgCl reference electrode.Oxygen was elimi- 12. A.M.Krstulovic and P.R.Brown.American nated from the system by purging the mobile phase Laboratory.May.17(1981). with N2 and by replacing all plastic tubes with (收稿日期:1983年9月1日) stainless steel tubes. Determination of 1-Nitropyrene’in Diesel Exhaust A nalysis of diesel samples indicated thepre- by HPLC with Reductive Electrochemical Detector sence of 1-NP at levels between 0.7 and 142 ng/mg JiZu-liany,Institute oEnvironmental Chemi- in 10 CH2Cl2 extracts.The identity of 1-NP was stry, Academia Rppapot t. confirmed by the comparison of both retention of BEHS,UC Berkeley,U.S.A. time and normalized hydrodynamic voltammo-

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