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PacBio RS II Sequencing System FIND MEANING IN COMPLEXITY Genome finishing • Epigenetics • Haplotype phasing Repeat expansions • Isoform Sequencing • Minor variants Extraordinary Read Lengths with the PacBio RS II ® The PacBio RS II sequencing system allows scientists to rapidly and cost effectively generate finished genome assemblies, reveal and understand epigenomes, and characterize genomic variation. It achieves the industry’s longest read lengths and highest consensus accuracy. Generate Finished Genomes The PacBio RS II finishes microbial genomes and improves assembly of larger organisms with multi-kilobase reads and unbiased coverage regardless of GC content. No amplification is required. Range of Genome Sizes Viruses / BACs Bacteria Lower Eukaryotes Higher Eukaryotes Benefits Plasmid 1 A B C A B C 1 1 110,366 bp A B B C A B’ C • Resolve mobile elements and Plasmid 2 2 2 2 2 structural-variation events 37,768 bp
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