文献_9-2013-Nucleotide-resolution DNA double-strand break mapping by next-generation sequencing.pdfVIP

Articles nucleotide-resolution dnA double-strand break mapping by next-generation sequencing 1,2,11,12 3,12 4 1,2,11 3,11 Nicola Crosetto , Abhishek Mitra , Maria Joao Silva , Magda Bienko , Norbert Dojer , 5,6 5,6 5–7 8 8 4 Qi Wang , Elif Karaca , Roberto Chiarle , Magdalena Skrzypczak , Krzysztof Ginalski , Philippe Pasero , Maga Rowicka3,9,10 Ivan Dikic1,2 We present a genome-wide approach to map dnA double-strand (ChIP-on-chip) or next-generation sequencing (ChIP-seq) has . breaks (dsBs) at nucleotide resolution by a method we termed been applied to map DSBs5–7. However, the fact that in ChIP- d e Bless (direct in situ breaks labeling, enrichment on streptavidin based methods DSBs are not directly labeled in situ, but rather v r and next-generation sequencing). We validated and tested Bless detected indirectly using antibodies targeting specific proteins e s e using human and mouse cells and different dsBs-inducing agents that bind to DSBs, represents a considerable source of bias. The r s and sequencing platforms. Bless was able to detect telomere phosphorylated histone variant H2A.X (γH2A.X) is typically used t h ends, sce endonuclease–induced dsBs and complex genome- as a marker of DSBs, but it can also mark single-strand breaks g i r