盐胁迫对油菜种子萌发的影响.doc

摘要 PAGE1 / II 摘要 本试验选用哈尔滨市农信油菜种子和黑龙江省盛鸿油菜种子为材料，在实验室内采用培养皿滤纸发芽法[1]，在不同浓度的NaCl单盐胁迫下进行种子发芽实验（浓度梯度设为0、0.2%、0.4%、0.6%、0.8%、1.0%、1.2%七个梯度），测定发芽率、发芽指数、发芽势和活力指数等指标。结果表明：在单盐胁迫下，随着胁迫浓度的增加，油菜种子的发芽率、发芽指数、发芽势和活力指数均有不同程度的降低，相对盐害率升高，种子开始发芽的时间推迟且发芽过程延长； NaCl的胁迫对油菜种子萌发的抑制作用很大，其中浓度0.4%NaCl的胁迫对油菜发芽率的抑制均达到了50%以上分别为88%、88%，在浓度为2.0%的NaCl胁迫下两种供试材料的发芽率均为零；盐胁迫对种子发芽有抑制作用，但低浓度盐胁迫对种子发芽有促进作用。农信油菜种子优于盛鸿油菜种子的抗盐性。 关键词：氯化钠胁迫；油菜；种子萌发 Abstract II/ II Abstract This experiment chooses its rape seed and Harbin in Heilongjiang ShengHong rape seed in the laboratory for material, using flasks filter method [1], germinate in different concentrations of salt stress were NaCl single seed germination experiment (concentration gradient for 0, 0.2%, 0.4% 0.6 percent, and seven gradient, 1.0%, 1.2%), the determination of germination rate, germination index, potential and vigor index, index. The results showed that the single under salt stress concentration of stress, along with the increase of seed germination, rape, germination index, germination and vigor index had different degrees, relative rate of salt, seeds to germinate time delay and germination process, The stress on rapeseed NaCl on seed germination, including the inhibition of the stress concentration of soybean NaCl 0.4% rate reached the inhibition of 50% respectively, concentration, 88% 88 for NaCl of 2.0% under the stress of two selected materials for the germination rate, Salt stress on seed germination, but low concentration of salt stress on seed germination. ShengHong rape seed than its rape seed salt resistance. Keywords: sodium chloride stress, Rape, Seed germination