建立口服耐受法治疗自身免疫性卵巢早衰.docVIP

 建立口服耐受法治疗自身免疫性卵巢早衰# 付莉1，冯卫2，崔松花1* （1. 吉林大学第二医院，长春 130041； 5 10 15 20 25 30 35 40 2. 吉林大学第一医院，长春 130021） 摘要：目的 通过口服透明带多肽 3（pZP3）诱导口服耐受来治疗自身免疫性卵巢早衰（POF）； 方法 将 100 只小鼠随机分为对照组、模型组、小剂量治疗组、中剂量治疗组和大剂量治疗 组。各治疗组小鼠给予透明带多肽灌胃，剂量分别为每次 1μg、10μg 和 100μg，共 7 次。灌 胃 4 次后，经皮下注射免疫原建立小鼠自身免疫性卵巢早衰模型。并观察各组小鼠的性周期、 ＋ ＋ 果各治疗组小鼠 POF 的发病率均较模型组降低，在中剂量治疗组中，小鼠的性周期规律， + + 论 本研究提示口服 pZP3 可能为诱导口服耐受、抑制 AOD 的发病提供一条新的治疗途径。 关键词：妇产科学；卵巢早衰；透明带；口服耐受 中图分类号：R711.75 Suppress autoimmune premature ovarian failure with oral tolerance FU Li1, FENG Wei2, CUI Songhua1 (1. Second Hospital of Jilin University, ChangChun 130041; 2. First Hospital of Jilin University, ChangChun 130021) Abstract: Objective To suppress autoimmune premature ovarian failure (POF) by oral administration of peptide of zona pellucida 3 (pZP3); Methods 100 mice were divided into control group, model group, low dose therapeutic group, middle dose therapeutic group and high dose therapeutic group randomly. The mice of therapeutic groups were fed with pZP3 by gastric intubation depending upon pZP3 concentration and tolerance protocol. To establish the animal model of POF, after 4 times of oral administration of pZP3, all the mice were immunized ＋ ＋ detected by flow cytometry, Serum and ovarian antibody to ovarian ZP were detected by immunofluorescence. Result After being treated, the incidence of POF in all therapeutic groups were lower than model group. In middle dose therapeutic group, estrous cycles were normalized, CD25+CD4+T cells increased. The severity of autoimmune oophoritis and the titer of ZP antibodies were also reduced. Conclusion Autoimmune POF can be suppressed by oral administration of pZP3. Keywords: gynecology; premature ovarian failure; zona pellucida; oral tolerance 0 引言 卵巢早衰（premature ovarian failure, POF）指卵巢功能提前衰退，表现为妇女 40 岁之前 出现闭经和潮热、失眠等更年期症状，实验室检查发现卵泡刺激素（follicle stimulating hormone, FSH）及黄体生成素（luteinizing hormone, LH）升高，而雌二醇下降，给患者带来 巨大的心理和生理上的痛苦。现阶段 POF 的治疗常仅以激素替代治疗和辅助生育技术为主， 这并不能从根本上治疗 POF，且常年应用还可能带来严重的副作用。因此，寻