颗粒物对卵清蛋白致敏豚鼠氧化损伤的研究.docVIP

 颗粒物对卵清蛋白致敏豚鼠氧化损伤的研 究# 梁丽君1，郭昌胜1,2，陈妍1，王春花1，王玉秋1** 5  摘要：目的 （1. 南开大学环境科学与工程学院，天津 300071； 2. 中国环境科学研究院，北京 100012） 研究不同颗粒物染毒后，卵清蛋白（OVA）致敏豚鼠氧化性损伤的情况。方 法 豚鼠分为单纯 OVA 致敏组、分别与三种颗粒物染毒的致敏染毒复合组和两个对照 组。豚鼠腹腔注射 10% OVA 1ml 致敏。对照组以生理盐水替代 OVA 行腹腔注射。距第一 10 15 20 25 30 35 40 次注射 21 天后，在密闭染毒箱中超声雾化给予 1% OVA，吸入激发。致敏后，部分动物在 密闭染毒箱中进行连续五天的颗粒物染毒，每天一小时。氧化性损伤研究的检测指标有超氧 化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH－PX）、丙二醛（MDA）。结果 OVA 致敏组和致敏染毒复合组，颗粒物染毒作用降低了 SOD 和 GSH－PX 活性，增加了 MDA 含量，但是染毒致敏复合组没有体现 OVA 的作用。结论 过敏原激发和颗粒物染毒相互作 用，会增强免疫反应程度，即颗粒物对过敏症有强效作用。但是在本实验模型中，过敏没有 体现使颗粒物引发的氧化性损伤进一步加剧的作用。 关键词：氧化性损伤；颗粒物；卵清蛋白；过敏；佐剂作用 中图分类号：R994.6 Oxidative Damage in OVA Sensitized Guinea Pigs which were Exposed to Different Kinds of Particulate Matter LIANG Lijun1, Guo Changsheng1,2, Chen Yan1, Wang Chunhua1, Wang Yuqiu1 (1. College of Environmental Scinece and Engineering,nankai university, Tianjin 300071; 2. Chinese Research Academy of Environmental Sciences, Beijing 100012, China) Abstract: Objective To study the Oxidative damage in OVA sensitized Guinea pigs which were exposed to different kinds of PM. Methods Guinea pigs were divided into OVA sensitized group, control groups and compound groups. Guinea pigs were sensitized with 0.1mg ovalbumin (OVA) in 1 ml in sterile saline injected intraperitoneally on the first day and the fifth day. Control groups received sterile saline. Twenty days later, all groups were challenged with an aerosol of 1% ovalbumin in sterile saline for 1 h in a 135 L chamber. After sensitization, mice were exposed in a 135 L chamber with different kind of PM and air on five consecutive days，with 1h per day. The activity of SOD and GSH －PX and the content of MDA were detected. Results DEP or OVA alone decreased intracellular superoxide dismutase and gluthatione peroxidase, and increased Malondialdehyde but without significant differences was observed when compared with PM exposure alone. Conclusion PM exposure had a adjuvant effect on enhancement of the sensitization. But allergen challenge and e