硫化镉量子点对中国仓鼠肺成纤维细胞（CHL）的毒性研究.docVIP

 硫化镉量子点对中国仓鼠肺成纤维细胞 （CHL）的毒性研究# 王红艳1,2，郭昌胜2，梁丽君1，王春花1，王玉秋1** 5 10 15 20 25 30 35 40 （1. 南开大学环境科学与工程学院，环境污染控制过程与基准教育部重点实验室， 天津 300071； 2. 中国环境科学研究院 北京 100012） 摘要：目的 研究不同浓度的硫化镉量子点（CdS QDs）对中国仓鼠肺成纤维细胞(CHL)的毒 性情况。方法 经乳化液膜法自主合成不同浓度的的硫化镉量子点，将 CHL 暴露其中进行 染毒。从细胞内外镉离子浓度、细胞氧化损伤情况（细胞内 MDA、SOD、GSH 含量）、细 胞膜损伤（乳酸脱氢酶-LDH 含量）等方面，推测细胞毒性机制。结果 随着染毒剂量的增 加，CHL 细胞存活率逐渐下降，细胞内 MDA 增加，而 SOD 和 GSH 有所下降，并呈现出 一定的剂量-效应关系。 结论 CdS QDs 的生物毒性可能是 Cd2+和细胞氧化损伤共同作用的 结果，同时也与脂质过氧化作用破坏细胞膜脂质双分子层的完整性增加了细胞膜通透性有 关。 关键词：量子点；氧化损伤；细胞毒性 中图分类号：R994.6 Cadmium Sulfide Quantum Dots induce Cytotoxicity in Chinese hamster lung fibroblasts (CHL) WANG Hongyan1,2, GUO Changsheng2, LIANG Lijun1, WANG Chunhua1, WANG Yuqiu1 (1. Key Labortory of Pollution processes and Environmental Criteria of Ministry of Eduation， College of Environmental Science and Engineering, Nankai University, Tianjin 300071; 2. Chinese Research Academy of Environmental Science, Beijing 100012) Abstract: Purpose To study the extent of cytotoxity which caused by various concentrations of self-made cadium sulfide quantum dots to Chinese hamster lung fibroblasts. Method Chinese hamster lung fibroblast (CHL) was exposed to different concentrations of CdS QDs which were synthesized by an emulsion liquid membrane (ELM) system. The parameters for evaluating the degree of cytotoxicity include cadmium ion concentration inside and outside cells, cell oxidative damage (cells MDA, SOD, GSH content) and membrane damage (LDH content), etc. Results Cell survival rates gradually decreased, MDA content increased while the SOD and GSH content declined yet showed a certain degree of dose - response relationship as the dose increased. Conclusion Biological toxicity of CdS QDs may not only via mechanisms involving both Cd2+ and oxidative damage, but also the role of lipid peroxidation and destruction of the bilayer lipid membrane integrity so that triggered an increase in the cell membrane permeability. Key words: quantum dots; oxidative damage; cytotoxicity 0 引言 作为一种