水稻根系响应铜胁迫的蛋白质组学研究.docVIP

 水稻根系响应铜胁迫的蛋白质组学研究# * 5 10 15 20 25 摘要：铜是植物生长发育所必需的微量元素，但是过量的铜对植物产生毒害作用。为理解水 稻对铜胁迫的响应机理，运用双向电泳和 MALDI-TOF/TOF MS 技术研究了铜胁迫对水稻幼 苗根系总蛋白质组的影响。结果显示，8 μmol/L CuSO4 处理的水稻幼苗与对照（0.32 μmol/L CuSO4）相比，显著差异表达（1.5 倍以上差异变化）的蛋白点有 34 个：包括抗氧化防御、 氧化调控、胁迫响应、硫和谷胱甘肽代谢、碳代谢和信号转导的蛋白及其它蛋白。这些氧化 还原、硫和谷胱甘肽代谢相关的蛋白可能与抗氧化酶协同建立一个新的稳态来响应铜胁迫。 关键词：水稻（Oryza sativa L.）；蛋白质组；铜胁迫；响应 中图分类号：Q945；Q5 Proteomic analysis of copper stress response in the roots of rice (Oryza sativa L.) SONG Yufeng, Zhang Hongxiao, SHEN Zhenguo, XIA Yan (Collge of Life Sciences of Nanjing Agricultural University, NanJing 210095) Abstract: Copper (Cu) is an essential micronutrient required for growth and development of plants. However, excess Cu is toxic to plants. To understand the mechanisms involved in copper stress response, a proteomic approach was used to investigate difference in Cu stress-induced protein expression of rice by two-dimensional PAGE and MALDI-TOF mass spectrometry. The results showed that thirty-four protein spots were found to be differently expressed in control (0.32 μmol·L-1 CuSO4) and Cu-treated (8 μmol·L-1 CuSO4) rice seedling. These identified proteins included those involved in antioxidative defense, redox regulation, stress response, sulfur and GSH metabolism, carbohydrate metabolism, signal transduction, and some other proteins with various functions. The majority of these proteins are involved in redox regulation and sulfur and GSH metabolism, suggesting that these proteins may work together with antioxidant enzymes to establish a new homeostasis in response to Cu stress. Keywords: Oryza sative L.; Proteomics; Copper stress; Response 30 0 引言 铜是所有植物和藻类生长发育所必需的微量元素。铜在植物光合、呼吸、氧化胁迫响应 和细胞壁代谢等过程中发挥重要作用[1]。然而过量的铜对植物是有毒害的，如抑制光合作用 和色素合成，产生氧化胁迫等。植物已经进化了几种应对重金属毒害的耐性机制，包括排斥、 35 40 区室化、螯合和与有机配体结合（如有机酸、氨基酸和多肽等）[2]。在重金属胁迫下，虽然 对不同植物进行了大量的生理生化分析；但是过量的铜对植物的影响还没有完全了解。 植物通过快速改变基因表达和蛋白合成对生物和非生物胁迫做出响应。了解重金属耐性 分子机制的方法之一是通过鉴定响应重金属胁迫的功能基因或蛋白[3]。最近，对重金属胁迫 下的水稻和其它植物进行了转录分析，以探究重金属胁迫下植物基因表达模式[4]。基于双向 电泳、质谱技术和生物信息学的蛋白质组