白草花叶病毒承德玉米分离物3’-cDNA 片段序列分析.docVIP

 白草花叶病毒承德玉米分离物 3′-cDNA 片段序列分析# 崔小雯1，高波2，许斐斐2，李向东3** 5 10 15 20 25 30 35 40 （1. 山东农业大学植物保护学院植物病理学系,泰安 271018； 2. 山东农业大学植物保护学院植物病理学系, 泰安 271018； 3. 山东农业大学植物保护学院植物病理学系, 泰安 271018） 摘要：采自河北承德 11 个表现矮花叶症状的玉米样品，用甘蔗花叶病毒(Sugarcane mosaic virus, SCMV)和白草花叶病毒(Pennisetum mosaic virus, PenMV)简并引物扩增了基因组 3′端 约 2.1kb 的片段并进行测序。Blast 结果表明其中 8 个样品含有 PenMV。扩增到的 PenMV 序 列均为 2 135 nt，包括部分 NIb 基因(985 nt)、完整的 CP 基因(909 nt)和 3′-UTR(241nt)。这 8 个分离物 CP 基因和 3′-UTR 与 GenBank 上其他 PenMV 分离物相应序列的核苷酸一致率分 别为 89. 8% ~93.4% 和 95. 9% ~ 97. 9% 。根据扩增的 2 135 nt 序列和 CP 基因序列构建系统 发育树，8 个分离物与 GenBank 上其他 PenMV 分离物都分为 2 个组：山西组和承德组。重 组分析表明 CD9 的 CP 基因存在重组。 关键词：白草花叶病毒；玉米；系统发育分析；重组 中图分类号：S435. 131; Q939. 46 Sequencing and analysis of the 3′-terminal genome of pennisetum mosaic virus isolates from maize plants in Chengde CUI Xiaowen1, GAO Bo2, XU Feifei2, LI Xiang-Dong3 (1. Department of Plant Pathology, College of Plant Protection,Shandong Agricultural University,Taian 271018, China; 2. Department of Plant Pathology, College of Plant Protection,Shandong Agricultural University, Taian 271018, China; 3. Department of Plant Pathology, College of Plant Protecton,Shandong Agricultural University, Taian 271018,China) Abstract: Totally 11 maize samples showing dwarf mosaic symptoms were collected from Chengde, Hebei Province. The 3′-terminal 2.1 kb genomic fragments were amplified with degenerate primers for Sugarcane mosaic virus (SCMV) and Pennisetum mosaic virus (PenMV) and then sequenced. The Blast results showed that eight samples were infected with PenMV. The cloned genome of these eight isolates were all 2 135 nucleotides (nt) long, including partial NIb gene (985 nt), complete CP gene (909 nt) and the 3′-UTR (241 nt). The CP gene and 3′-UTR shared nt identities of 89. 8% - 93. 4% and 95. 9% - 97. 9% , respectively, with the corresponding PenMV sequences available in the GenBank. In the phylogenetic trees constructed with the 3′ -terminal 2135 nt sequence and CP gene, all the PenMV isolates were divided int