鲍鱼腹足肌酸性多糖的分离纯化、组成分析及其体外抗肿瘤活性研究.docVIP

 鲍鱼腹足肌酸性多糖的分离纯化、组成分析 及其体外抗肿瘤活性研究# 李国云，杜磊，常耀光，薛勇，王玉明，薛长湖** 5 10 15 20 25 30 35 （中国海洋大学食品科学与工程学院，青岛 266003） 摘要：本文对鲍鱼腹足肌酸性粘多糖（APP）进行提取、分离纯化及基本组成分析，并研究 其体外抗肿瘤活性。鲍鱼腹足肌经木瓜蛋白酶酶解提取、CPC 沉淀、盐溶、乙醇沉淀、透 析、冻干得到鲍鱼腹足肌粗多糖，经离子交换层析和凝胶过滤层析后得到纯度较高的一种新 型鲍鱼腹足肌酸性多糖 AAP。经鉴定其平均分子量为 56.2kDa；柱前衍生高效液相色谱法分 析单糖组成，绝大部分为氨基半乳糖（GalN）、葡萄糖醛酸（GlcUA）、岩藻糖（Fuc）和 半乳糖（Gal），其摩尔比分别为 2.14:2.37:2.97:1，此外还均含少量鼠李糖（Rha）和氨基葡 萄糖（GlcN）；离子色谱法测定硫酸基含量为 15.5%。体外抗肿瘤实验表明 AAP 能够显著 抑制小鼠 S-180 肉瘤细胞和人肝癌细胞 HEP-G2 的增殖。 关键词：水产品贮藏与加工；鲍鱼；酸性多糖；分离纯化；化学组成；体外抗肿瘤 中图分类号：S912 Isolation, chemical composition and antitumor activity in vitro of the acid polysaccharide isolated from Haliotis discus hannai Li Guoyun, Du Lei, Chang Yaoguang, Xue Yong, Wang Yuming, Xue Changhu (College of Food Science and Engineering, Ocean University of China, Qingdao 266003) Abstract: A novel glycosaminoglycan-like sulfated polysaccharide (AAP) from the pleopods of Haliotis discus hannai Ino was purified by DEAE ion exchange chromatography followed with S-300 HR geltrion chromatography. Chemical composition analysis indicated that AAP was composed of galactosamine, glucuronic acid, fucose, galactose with a ratio of 2.14:2.37:2.94:1, the content of sulfate was 15.5%. The average molecular weight (Mw) was 56.2 kDa in a TSK-gel G4000PWXL column. The antitumor activity of the sulfated polysaccharide in vitro was detected by MTT assay. It was found that AAP had an obvious antitumor activity in a dose-dependent manner in vitro and the maximum inhibitory ratio of S-180 cells and Hep-G2 cells were 52.5% (800 μg/mL) and 48.9% (800 μg/mL), respectively. Key words: storage and processing of aquatic products；abalone; acid polysaccharide; purification; chemical composition; antitumor 0 引言 鲍鱼（Haliotis spp.）属软体动物门，腹足纲，鲍科，为藻食性动物，营养价极高， [1] 道鲜美，鲍鱼肉含有丰富的球蛋白，本身营养价值极高。鲍鱼多糖是重要的生理活性物质之 [2-3] [4-5] 40  [6] [7] 基金项目：教育部博士点基金（No.200804230008）；国际科技合作项目(2010DFA31330) 作者简介：李国云(1987- )，女，博士研究生，研究方向：海洋生物活性物质 通信联系人：薛长湖(1964- )，男，博士，教授，研究方向：水产化学；E-mail: xuech@ouc.edu.cn. E-mail: xu