三种乳化剂对厚朴酚抑制变形链球菌生长及产酸作用影响的体外研究.docVIP

 三种乳化剂对厚朴酚抑制变形链球菌生长 及产酸作用影响的体外研究# 刘琳1,2，包广洁2** 5 10 15 20 25 30 35 40 45 （1. 兰州大学口腔医学院，甘肃 兰州 730000； 2. 西北民族大学口腔医学国家民委重点实验室，甘肃 兰州 730030） 摘要：目的：考察厚朴酚在乳化剂聚乙二醇 400、吐温 20、吐温 80 增溶作用下对变形链球 菌生长及产酸的抑制情况，研究乳化剂对厚朴酚抑菌性的影响，并对原因进行分析。 方法：选用变形链球菌临床分离菌株，用 MS（轻唾-琼脂培养基）培养细菌。1 将厚朴酚分 别溶于 40%的聚乙二醇 400、吐温 20、吐温 80 溶液中并用相同乳化剂溶液将其等比稀释至 预设浓度，采用纸片法将它们同时平行作用于变形链球菌做抑菌试验，比较上述厚朴酚于乳 化剂混合物的抑菌效果。2 将厚朴酚分别溶于三种乳化剂并用 MS 液体培养基将其等比稀释 至预设浓度，将等量的菌液加置培养基中，在特定条件下培养后考察三组药物在乳化剂作用 下抑止细菌产酸的效果。 结果：1.在高浓度时厚朴酚与聚乙二醇 400 作用后抑菌效果好，而在低浓度时药物与吐温 80 作用后抑菌效果好。2.厚朴酚与聚乙二醇 400 作用后对变形链球菌产酸能力抑制作用最强， 与乳化剂吐温 80 作用后对变形链球菌产酸能力抑制作用最弱。 结论：在不同乳化剂增溶作用下厚朴酚抑制变型链球菌生长及产酸效果有差异，乳化剂中亲 油基链的增长增加了对厚朴酚扩散的抑制作用。 关键词：厚朴酚；乳化剂；抑菌性；变形链球菌；产酸 中图分类号：R781 In vitro studies of influence of three kinds of emulsifiers on inhibitory effect of magnolol on growth and acid production of streptococcus mutans Liu Lin1,2, Bao Guangjie2 (1. School of Stomatology, Lanzhou University, Lanzhou 730000, China; 2. Key Lab. of Stomatology of State Ethnic Affairs Commission, Northwest University for Nationalities, Lanzhou 730030, China) Abstract: Objective：To study the influence of emulsifiers on inhibitory effect of the magnolol on streptococcus mutans and its mechanisms by studying the inhibitory effects of magnolol on the growth and acid production of streptococcus mutans under solubilization of three emulsifiers - polyethylene glycol 400, Tween20, Tween80. It can be a reference to selecting emulsifier when the anti-caries drug prescription can be made in Magnolia. Method: Magnolol was dissolved in 40% solution of three kinds of emulsifier, and proportionally diluted to the preset concentration with solution of emulsifier. Then the effects of the agent on the growth of the bacteria were assessed by slip diffusion method. 2. Magnolol was dissolved in 40% solution of three kinds of emulsifier, and proportionally diluted to the preset concentration with MS liquid media. The same amount of bacteria was added into the MS liquid medium and was cultured to study the inhib