人宫颈癌组织中Sema4C和Plexin-B2的表达与淋巴转移的关系.docVIP

 人宫颈癌组织中 Sema4C 和 Plexin-B2 的表 达与淋巴转移的关系# 吴明富，马全富，杨洁，马丁，黎宁，刘丽江** 5 10 15 20 （华中科技大学同济医学院附属同济医院） 摘要：目的: 检测 Sema4C 和 Plexin-B2 蛋白在人宫颈癌组织中的表达，探讨其与宫颈癌组 织学类型、临床分期、病理分级和淋巴转移的关系。方法: 采用免疫组织化学 SP 法和半定 量方法检测 Sema4C 和 Plexin-B2 蛋白在 118 例宫颈癌组织 (有淋巴结转移者 23 例和无淋巴 结转移者 95 例)和 28 例正常宫颈组织中的表达。结果: Sema4C 和 Plexin-B2 蛋白在宫颈癌组 织 LECs 和癌细胞中的阳性表达率分别为 72.88％和 74.58％，明显高于正常宫颈组织（均 P 0.01）；在不同组织学类型和年龄中表达差异无统计学意义（均 P 0.05）；期阳性表达 率分别为 67.53％和 71.43％,明显低于期者 80.49％和 85.37％（P 0.05）；有转移者阳性 表达率 82.61％和 86.96％明显高于无转移者 34.74％和 40.00％，两者差异均有显著性意义 (P0.05)。结论: Sema4C 和 Plexin-B2 蛋白的表达与临床分期、分化程度及淋巴转移等密切 相关，提示 Sema4C/Plexin-B2 信号通路在人宫颈癌淋巴转移中起重要作用。 关键词：信号素蛋白；4C Plexin-B2； 宫颈癌； 淋巴转移； 免疫组织化学 中图分类号：R737 The expression and significance of Sema4C and Plexin-B2 in the human cervical carcinoma tissues and its correlation to lymphatic metastasis WU Mingfu, MA Quanfu, YANG Jie, MA Ding, LI Nin, LIU Lijiang (Department of Obstetrics and gynecology, Tongji Hospital, Tongji medical college, Huazhong 25  Abstract: university of science and technology) Objective To investigate the expression of Sema4C and Plexin-B2 in human cervical cancers and to explore the correlation between the expression of Sema4C and Plexin-B2 and the malignant status, including histological types, clinical stage, pathological grade, and lymphatic metastasis. 30 35 40 Methods Immunohistochemistry （SP method）and semiquantitative analysis were used to measure the expression of Sema4C and Plexin-B2 in 118 cases of human cervical cancers and 28 cases of human normal cervixes. Results Sema4C and Plexin-B2 protein expressed in 72.88％ and 74.58％ of patients with human cervical cancer respectively, whose expression were higher than those in human human normal cervixes, suggesting that the difference reached statistical significance (P 0.05). There was no obvious difference in the expression of Sema4C and Plexin-B2 among the histological types and years of age of human ovarian carcinomas. The positive expression levels of Sema4C and Plexin-B2