三苦滴丸对麻醉犬冠脉结扎致心肌缺血的保护作用.docVIP

 三苦滴丸对麻醉犬冠脉结扎致心肌缺血的 保护作用# 尚坤，李娜，隋殿军** 5 10 15 20 25 30 35 40 （长春中医药大学药学院，长春 130117） 摘要：目的：研究三苦滴丸对麻醉犬冠脉结扎致心肌缺血的保护作用。方法：采用麻醉开胸 犬冠状动脉左前降支结扎造成急性心肌缺血模型，将 30 只比格犬随机分为模型组，阳性对 照组及三苦滴丸高、中、低剂量组，每组 6 只。观察给药后犬血清肌酸激酶（CK）、乳酸 脱氢酶（LDH）、天冬氨酸氨基转移酶（AST）水平的变化。通过 N－BT 染色，观察心肌 梗死的范围。并测定冠状静脉窦及动脉血氧分压，观察心肌耗氧量的变化。结果：三苦滴丸 组犬血清中 CK 的浓度较其余组显著降低，心肌梗死范围较其他组显著缩小，心肌耗氧量下 降（P﹤0.05，P﹤0.01）。结论：冠三苦滴丸能明显减轻犬心肌梗死时的心肌缺血程度，从 而保护心肌。 关键词：中药药理；三苦滴丸；心肌缺血；冠脉结扎 中图分类号：R285.5 Protection of sanku dropping pills for myocytes in anesthetic dog with myocardial ischemia caused by coronary artery ligation Shang Kun, Li Na, Sui Dianjun (Changchun University of Traditional Chinese Medicine, ChangChun 130117) Abstract: Objective: It is to study the protection of sanku dropping pills for myocytes in anesthetic dog with myocardial ischemia caused by coronary artery ligation．Methods: The acute myocardial ischemic canine models were established by left anterior descending artery ligature after opening thoracic cavity under anesthesia．Totally 30 Beagle dogs were randomly assigned to five groups， which were the model group，danshen group，and sanku dropping pills groups with large，median， and small doses of the drugs．The concentration changes of serum CK，LDH and AST were observed and the infarct size was measured by N－BT dyeing．Results: The concentrations of CK in the sanku dropping pills groups were significantly lower than that in the other groups，as well as the infarct size significantly smaller than that in the other groups．sanku dropping pills could decrease myocardial oxygen consumption significantly. Conclusion: sanku dropping pills could protect the myocytes in the ischemic canine preparation as demonstrated by the smaller infarct size and lower concentration of biomarkers. Keywords: pharmacology of Chinese medicine; sanku dropping pills; myocardial ischemia; coronary artery ligation 0 引言 三苦滴丸是以三七和苦碟子等为主要成分研制而成的新中药制剂，三七、苦碟子均具有 活血通脉、化瘀止痛等功效，可以扩张冠脉，增加冠脉血流量，保护心肌，单用治疗冠心病 的研究很多，并有较显著的疗效。但是二药配伍及其复方制剂研究尚处于空白。本实验将二 者提取物进行合理配制，