人工蛹虫草中N6-（2-羟乙基）腺苷的分离纯化及其对小鼠lewis肺癌细胞的增殖抑制作用的研究.docVIP

 人工蛹虫草中 N6-（2-羟乙基）腺苷的分离 纯化及其对小鼠 lewis 肺癌细胞的增殖抑制 作用的研究# 5 10  * 摘要：N6-（2-羟乙基）腺苷是第一个生物来源的钙离子拮抗剂，具有很强的抗紫外辐射、 抗血小板凝集和镇痛等效用。关于其抗肿瘤方面的作用报道很少。本研究采用水提醇沉、制 备液相分离和结晶等简单的方法，从蛹虫草水提取物中分离出了 N6-（2-羟乙基）腺苷。并 利用小鼠 lewis 肺癌细胞为模型，采用 MTT 法对其体外抗肿瘤效果做了初步研究，发现它对 小鼠 lewis 肺癌细胞具有较强的抑制作用，且此种抑制作用呈时间-剂量依赖效应。此为探 索和拓展 N6-（2-羟乙基）腺苷的其他功效提供了一定的借鉴作用。 15 关键词：生物化学；N6-（2-羟乙基）腺苷；蛹虫草；抗肿瘤 中图分类号：Q58 Study on the Isolution and Purification of 20 N6-(2-Hydroxyethyl)adenosine from Cordyceps militaris and Its Inhitory Effect on the Lewis Lung Cancer LU Lili1, SHU Tejun1, CHEN Jianqing1, WU Xiangfu1, ZHANG Yaozhou1,2 (1. Institute of Biochemistry,College of Life Science,ZheJiang Sci-Tech University,HangZhou 310018; 25  Abstract: 2. TJAB TianJin 300457) N6-(2-Hydroxyethyl)adenosine is a kind of calcium antagonist of the first biological sources. It has strong resistance to UV radiation, and the effectiveness of anti-platelet aggregation and analgesic. Little is reported about its role in the anti-tumor. Method of water extraction and ethanol precipitation, preparative liquid chromatography and crystallization was used to isolate 30 35 40 and purify N6-(2-Hydroxyethyl)adenosine in this study. MTT assay was used to preliminary investigated the inhibitory effect of N6-(2-Hydroxyethyl)adenosine on tumor in vitro while the Lewis lung cancer was used as model. Result showed that N6-(2-Hydroxyethyl)adenosine had a relatively strong inhibitory effect on Lewis lung cancer with a time-dose depend effect. This study provided a certain reference for the exploration and expansion of the function of N6-(2-Hydroxyethyl)adenosine. Key words: biochemisty; N6-(2-Hydroxyethyl)adenosine; Cordyceps militaris; anti-tumor. 0 引言 蛹虫草[Cordyceps militaris (L.ex.Fr) Link]，又名北冬虫夏草，蛹草，北虫草，蛹草菌等， 分类学上属于真菌门(Eumycota)，子囊菌亚门(Ascomycotinia)，核菌纲(Pyrenomycetes)，球壳 目(Sphaeriales)，麦角科(Claviciptiaceae)，虫草属(Cordyceps) [1]。其与冬虫夏草同属异种， 是国内外公认的食药用真菌，民间用于肺炎、肾虚、腰痛等疾病的治疗[2]。研究表明蛹虫草 -1-  具有与冬虫夏草极相似的作用，无毒副作用，可以作为冬虫夏草的代用品[3]，其在药用价值 方面有着巨大的开发潜力