人帽状期牙胚的基因芯片分析.docVIP

 人帽状期牙胚的基因芯片分析# * 5 10 15 20 25 30 35 40 45 摘要：在哺乳动物牙齿发育中，众多基因发挥着重要作用。为了揭示调节人类牙齿发育中的 分子机制问题，我们对发育到帽状期的人磨牙、门牙、切牙的牙胚以及唇部进行了基因芯片 分析。利用 R 软件对基因芯片的数据归一化处理后，在 NIA 芯片分析程序中进行主成份分 析和聚类分析。主成份分析结果显示门牙和尖牙被分成一类，而磨牙，唇各分为一类，聚类 分析结果验证了主成份分析结论，并且总结列出了在各个牙齿中特异性高表达的基因。同唇 部相比，在牙胚组织中高表达的基因有 256 个。针对这些基因进行了 Go 基因注释分析，结 果显示牙胚中高表达基因的功能主要与细胞外基质的构成、细胞连接、Wnt 蛋白的结合、序 列特异性 DNA 的结合、胚胎器官发育、上皮细胞与间充质的信号传导，调节转录，调节磷 酸化等生物学过程密切相关。通过文献检索分析，发现牙胚高表达基因中的部分基因与哺乳 类动物的牙齿缺陷和发育异常相关。本研究为理解人类牙齿发育的分子机制提供实验数据。 关键词：器官发育；牙齿发育；基因芯片；GO 基因注释分析 中图分类号：Q954.48 Genome-wide analysis of gene expression in the Cap Stage of Human Embryonic Tooth Germ Huang Zhen, Hu Xuefeng, Zhang Yanding (College of Life Sciences, Fujian Normal University,Fuzhou 350108) Abstract: Numerous genes have been identified to play important regulative role during tooth development in mice. However, very little is known about gene expression and function in odontogenesis in humans. The aim of this research is to survey gene expression profile in early developing human tooth. We conducted microarray assay to examine gene expression in the incisor, canine, and premolar in human embryos of 12-week of gestation.The R statistical environment and the NIA Array Analysis were used to perform data analysis. First, the raw microarray data was corrected, filtered and normalized by affy package in the R statistical environment. And then PCA (Principal Component Analysis) and HCA (Hierarchical clustering Analysis) were applied to clustering microarray datasets. The odontogenic genes screening from the HCA were applied to Gene Ontology analysis by BINGO (a Cytoscape’s plugin).We identifed 256 genes that are highly expressed in the developing tooth as compared to that in the lip tissues. Functional categorization of those genes revealed multiple geneontology classes that included extracellular region, sequence-specific DNA binding, Wnt-protein binding, system development, organ morphogenesis, cell differentiation et al. we also found a great part of genes are r