NF-kB抑制剂PDTC在柯萨奇B3病毒引起的病毒性心肌炎小鼠中的作用机理研究.docVIP

 NF-kB 抑制剂 PDTC 在柯萨奇 B3 病毒引起 的病毒性心肌炎小鼠中的作用机理研究# * 5 10 15 20 25 30  摘要：目的 本文的研究目的是通过研究 PDTC 在科萨奇 B3 病毒引起的病毒性心肌炎中的 作用，探讨 NF-kB 在病毒性心肌炎中的病理机制。方法 本文采用 CVB3 病毒腹腔感染四 周龄雄性 Balb/c 小鼠的方法，建立病毒性心肌炎体内模型。在此模型上，检测了 NF-kB 的 抑制剂，PDTC，对 CVB3 引起的心肌炎小鼠的死亡率、心指数、射血分数、心肌炎性浸润 程度和血清中细胞因子表达水平的影响. 结果 实验结果表明, PDTC 能减少病毒性心肌炎 小鼠的死亡率，降低其心指数，显著降低病毒引起的心肌炎性浸润，抑制病毒性心肌炎小鼠 TNF-α 和 IL-17 的表达，促进 IFN-γ 的表达。结论 以上结果提示，NF-kB 信号通路和 IL-17 信号通路确实参与了病毒性心肌炎的炎症反应调控。 关键词：病理学；PDTC；NF-kB；病毒性心肌炎；科萨奇 B3 病毒 中图分类号：R365 Mechanisms of NF-kB inhibitor, PDTC, in mice with viral myocarditis induced by Coxsackievirus B3 ZHANG Yuanyuan (Department of Pharmacology, West China School of Preclinical and Forensic Medicine, Sichuan University, ChengDu 610041) Abstract: Objective The aim of the present study was to explore pathological mechanisms of NF-kB in viral myocarditis through investigating effects of NF-kB inhibitor, PDTC, on mice with viral myocarditis induced by CVB3. Methods All together, 60 male Balb/c mice was randomly divided into 4 groups, normal control group, PDTC group, CVB3 group, and CVB3+PDTC group. Viral myocarditis was induced by peritoneally injection of CVB3. PDTC was orally administered at a dosage of 37 mg/kg consecutively for 14 days. Mortality, heart indexes, cardiac functions, infiltration, and expressions of some cytokines were measured to elucidate effects of PDTC on viral myocarditis induced by CVB3.Results It was observed that PDTC can decrease mortality, enhance ejection fractions, increase expressions of IFN-γ, and decrease expressions of TNF-α and IL-17 in serum of mice with viral myocarditis induced by CVB3. Conclusions NF-kB pathway and IL-17 pathway did play roles in viral myocarditis induced by CVB3, which may serve as therapeutic targets. Keywords: Pathology; PDTC; NF-kB; Viral myocarditis; Coxsackievirus B3 35 0 引言 简病毒性心肌炎是由多种病毒引起的心肌局限性或弥漫性炎症，其临床表现大多可在 1-2 周内有发热、咽痛等类似上呼吸道感染症状，随后出现胸闷、心悸、疲乏、心前区隐痛、 气促等，严重时可并发心力衰竭。近年来，病毒性心肌炎在我国的发病率迅速增加，尤其好 40 发于儿童及青壮年，已成为严重损害国人健康的新的常见病。已证实，科萨奇 B3 病毒 （