HPLC-动力学理论讲座.ppt

Kinetex™Core-ShellTechnology

State-of-the-artParticleTechnology

forIncreasingLCProductivityandAchievingOptimal

HPLC/UHPLCPerformance

Traditional3.5µmHPLCcolumnCo-elution

Kinetex™Technology,SameColumnSizeandConditions

6.5minutes

FullyResolved6minutes

FullyResolved1.5minutes!

Kinetex™TechnologyShorterColumnIncreasedFlowRate

Kinetex™-WhattoExpect

Kinetex™Core-ShellTechnology

SectionI:AdvancedTechnologyforUltra-HighEfficiencyHPLC

SectionII:Kinetex™Core-ShellTechnologyPerformance

SectionIII:TheProductiveLaboratory-Kinetex™TechnologyInPractice

AnalyticalChallenges–DoMore,Faster,withLess

Capitalbudgetsreducedorfrozen

Operatingbudgetsbeingdownsized

Maintainingcompetitiveedge

Costofmanufacturing/QC

Costofdiscovery/development

Timetomarket

Fastcontractanalyticalservices

Costpersample

Fast,efficienttechnologytransfer

GlobalHealthConcerns

Fastandaccurateanalysisforcontaminantsinfoodandtheenvironment

Solution:Ultra-HighEfficiencyHPLC

Abilitytoshortenanalysistimeswithoutsacrificingresolution

Moreproductiveanalyticallaboratory

Lowerorganicsolventconsumption

Greaterpeakheightsforimprovedsensitivity

Lowerlimitsofquantitation(LOQ)forsampleanalytes

Lowerlimitsofdetection(LOD)forsampleanalytes

Betterresolutionforcomplexormulti-componentmixtures

Narrowpeakwidths,morebaselineresolvedanalytesperunitlength

HigherProductivity･BetterQualityData･LowerOperatingCosts

TheQuestforHigherEfficiency-PlateCount(N)

EfficiencyorPlateCount(N)

Themeasureof“TheoreticalPlates”inagivenlengthofcolumn

N=

w1/2

tR

Ultra-HighEfficiencyTechnologies

Narrowerpeakwidths

Greaterpeakheight

Increasedresolution

2

5.54

PlateHeight(H)

–or–

HeightEquivalenttoaTheoreticalPlate(HETP)

Newer,advancedHPLCcolumntechnologiesseektoachieveveryhighplat