天柏金黄散的药效学评价.docxVIP

天柏金黄散的药效学评价

【摘要】目的：评价天柏金黄散对小鼠模型的抗炎镇痛作用以及对膝骨关节炎大鼠模型的影响，为该医院制剂的临床推广应用提供实验依据。方法：采用2种小鼠抗炎试验（耳肿胀及毛细血管通透性试验）和2种小鼠疼痛模型（醋酸扭体模型、甲醛致痛模型）评价天柏金黄散的抗炎镇痛作用；然后采用注射木瓜蛋白酶合半胱氨酸（2:1）方法建立大鼠膝骨关节炎模型，造模成功后将大鼠随机分为模型组、阳性对照氟比洛芬组、天柏金黄散组，另设正常对照组，各组给予相应药物或干净纱布捆绑连续14d，定期测定大鼠膝关节直径，末次给药后1h，取大鼠软骨进行HE染色观察形态学改变并进行Mankin评分。结果：天柏金黄散对醋酸诱发的小鼠扭体反应和甲醛致痛均有显著的镇痛作用(P0.01)；并明显抑制二甲苯引起的小鼠耳肿胀(P0.05)、醋酸诱导毛细血管通透性增加(P0.01)；与模型组比较，天柏金黄散能显著改善KOA大鼠模型的膝关节肿胀程度和改善软骨病变(P0.01)。结论：天柏金黄散具有显著的抗炎镇痛作用，能明显改善KOA大鼠模型及软骨组织病理情况。

【关键词】天柏金黄散；药效评价；膝骨关节炎；抗炎；镇痛

PharmacodynamicevaluationofTianbaijinhuangsan

[Abstract]Objective:Toevaluatetheanti-inflammatoryandanalgesiceffectofTianbaiJinhuangsanonmousemodelsandtheeffectonratmodelsofkneeosteoarthritis,whichprovidedexperimentalbasisfortheclinicalpromotionandapplicationofpreparationsinthishospital.Methods:Twomouseanti-inflammatorytests(earswellingandcapillarypermeabilitytest)andtwomousepainmodels(aceticacidtwistingmodelandformaldehydepainmodel)wereusedtoevaluatetheanti-inflammatoryandanalgesiceffectsofTianbaiJinhuangsan.Then,themodelofkneeosteoarthritisofratswasestablishedbyinjectionpapain-combinedcysteine(2:1),andaftersuccessfulmodeling,theratswererandomlydividedintomodelgroup,positivecontrolflurbiprofengroup,TianbaiJinhuangsangroup,andanothernormalcontrolgroup,eachgroupwasgiventhecorrespondingdrugorcleangauzebundlefor14days,thekneejointdiameterofratswasmeasuredregularly,and1hafterthelastadministration,ratcartilagewastakenforHEstainingtoobservemorphologicalchangesandMankinscore.KMmicewererandomlygroupedaccordingtobodyweight,andthemicewereadministeredcontinuouslyfor7days,andthemiceweresubjectedtoacetatetwisting,formaldehydepain,earswellingandcapillarypermeabilityexperimentstoevaluatetheanti-inflammatoryandanalgesiceffectofTianbaijinhuangsan.Results:TianbaiJinhuangsanhadsignifican