BioEdit多序列比对及结果导出.docxVIP

1、 打开软件BioEdit 1、 打开软件 2、 点击 File open 打开需要进行比对的多序列 3、 点击 Edit select all sequence 4、击 Accessory Application Clustalw Multiple alignmen ClustalW Opt io ns R Btoronco:  ClustalW Multicle alianmen1 T hom pso, n J ,D. H ig gin, s D.G. ond Gib s o,n T.J. (1994) CLUST ALW improving the sensitrvit.Y of progressive multiple seq uence a.lig nment 1hroug h sequence v,ie ig htin,g gap pena l1ie 它 a nd weight mEt.t tix ch o ic e N ud e ic Acids Reseorch, submitte d, June 1 994 P- F u ll M ul iip l e alignment 广 Calcula1.e N J Tree p osition specific 厂FAST oJg orithm io r g uide tree P Bootstra. NJ Tree- N umb er of b oo tstra p s: (10 00 ties: Blank=d efault nmen1s Multiple eJi g n meni 广- Ga p op gn「一 d 厂— Ga p 或 end 厂－ 二Other P己r邸 eters: I 二 e nter a.d ditio n刮 pa rarneters a心 SIngIe In e ...... ......... ........ ........一............... ...... .... .........畴..... ...一................. .........一......... ......................... ... F !ou u t Clusto.1 totm o.t w ith Clusfo.1 consensus sequenee gener o.fi on\ · ····-······-·- ····一.... 巳eter s for Cluste lW 垒 巳 必 enernl settings:i:kkk } QUICKTR EE ·use F AST algorithm for th e alignmen 1 guide tree )NE\,可 REE;;;; :f 仆e for new guide tree }U 8 ETR EE= ．fi|巳 fo「 口Id guid巳 打己巳 INEGAT IVE :protein al19 nme nt wi1h ne g ativ e v alues in ma trix 可 ．｀｀＿且R.un O u3to|W l V ie w C|ust巳IW D oc | Co.no引 ．｀ ｀ ＿ BioEdit Seq uence Alig nment Ed it o r A log file is b eing g e ner at ed. It will be locat ed in D:\ Te mp . The log fi le for this se ssion will be call ed Bio Edit _clu stalw_071 91 6_210213.log. The temporary input file for this run will be called - fast a_07 1 916_210213.t m p. The temporary output file will be call ed ~ ali g n_071 916_210213.tmp. Run parameters will be in a file called clusParm _0 71916_210213.tx t. All of these files will be locat ed in D:\ Tem p N ot e: Th ese files wiil not be deleted aut o m at ica ll y. Yo u may want to dean them out of your swap direc1ory p eriodically. NOTE T H E FOt