SWCNT对AECⅡ的细胞毒性及机制研究的中期报告.docxVIP

SWCNT对AECⅡ的细胞毒性及机制研究的中期报告 SWCNT对AECⅡ的细胞毒性及机制研究的中期报告 摘要： 本研究旨在探究单壁碳纳米管（SWCNT）对肺泡Ⅱ型上皮细胞（AECⅡ）的细胞毒性及其可能的机制。本研究采用体外细胞实验，将不同浓度的SWCNTs与AECⅡ共培养，使用CCK-8和DHE染色来评估SWCNTs对细胞增殖和氧化应激的影响。结果显示，SWCNTs能够抑制AECⅡ的细胞增殖，同时增强细胞的氧化应激反应。此外，我们还发现SWCNTs可以导致细胞内钙离子浓度增加，激活细胞内信号通路。这些结果表明，SWCNTs能够诱导AECⅡ细胞的毒性作用，可能是与SWCNTs诱导的氧化应激反应和细胞内信号通路的激活有关。 关键词：单壁碳纳米管；肺泡Ⅱ型上皮细胞；细胞毒性；氧化应激；细胞内信号通路 Abstract: The aim of this study is to investigate the cytotoxicity of single-walled carbon nanotubes (SWCNT) to alveolar epithelial cells type II (AECⅡ) and its possible mechanism. In this study, in vitro cell experiments were conducted. Different concentrations of SWCNTs were co-cultured with AECⅡ, and CCK-8 and DHE staining were used to evaluate the effects of SWCNTs on cell proliferation and oxidative stress. The results showed that SWCNTs could inhibit the proliferation of AECⅡ and enhance the oxidative stress response of cells. In addition, we also found that SWCNTs can cause an increase in intracellular calcium ion concentration and activate intracellular signaling pathways. These results indicate that SWCNTs can induce the toxic effects of AECⅡ cells, which may be related to the induction of oxidative stress reaction and the activation of intracellular signaling pathways by SWCNTs. Keywords: single-walled carbon nanotubes; alveolar epithelial cells type II; cytotoxicity; oxidative stress; intracellular signaling pathways.