Agilent Polystyrene Standards on Agilent PLgel 3 μm MiniMIX-E using Gel Permeation Chromatography 说明书用户手册.PDF

Polystyrene Standards on Agilent PLgel 3 µm MiniMIX-E using Gel Permeation Chromatography Technical Overview Introduction Gel permeation chromatography using Agilent PLgel 3 µm MiniMIX-E narrow bore columns easily resolves polystyrene Mw 580 into individual oligomers. 1 5 2 Key 3 1. 34,500 2. 9,200 3. 3,250 4. 580 4 5. 162 6 min 20 Figure 1. Separation of low molecular weight polystyrene standards on Agilent PLgel 3 µm MiniMIX-E columns. Conditions Column 2 × Agilent PLgel 3 µm MiniMIX-E, 250 mm × 4.6 mm (p/n PL1510-5300) Eluent THF Flow Rate 0.3 mL/min Detector UV, 254 nm System Agilent PL-GPC 50 Agilent PLgel 3 µm MiniMIX-E columns Agilent PLgel MIXED-E, with its high efficiency ( 80,000 plates/m) and broad resolving molecular weight range (up to 30,000 daltons relative to polystyrene), is the preferred packing for low molecular weight prepolymers. The PLgel MiniMIX-E version is valuable in reducing solvent use by 70% when compared to the PLgel MIXED-E version. GPC/SEC columns and calibrants from Agilent Agilent offers a comprehensive portfolio of GPC/SEC columns and calibrants for high performance separations based on molecular size in solution. Agilent delivers leading solutions for characterizing and separating polymers by GPC/SEC, and manufactures all components for accurate polymer analysis. Look at the Agilent Literature Library on /chem/gpc-sec for a com- prehensive range of application notes and technical overviews to help you get the best from your Agilent GPC/SEC columns and i