毕业论文·设计《论文-酒精对小白鼠肝脏的损伤及其机理研究》.docVIP

论 文 作品名称： 酒精对小白鼠肝脏的损伤及其机理研究 摘 要 目的:对酒精对小白鼠肝脏的损伤及其可能产生的影响机理进行研究.方法:用体重为18-22g的小白鼠40只,随机分为四组.1，2，3组分别用10%,30%和50%的酒精灌，每天一次；第4组为对照组，用0.9%的生理盐水进行灌胃，每天一次，持续5wk。每周测量和记录一次各组小白鼠的体重，观察和记录小白鼠的精神状态， 同时测量小白鼠的血清ALT、AST的含量。5wk后，处死小白鼠，取每只小白鼠相同部位的肝脏，观察其形态。结果:(1)各组小鼠平均体重均有增加，第1组增加最多，第3组增加最少;(2) 第1，4组小白鼠食欲正常，精神状态好，第2，3组小白鼠有醉酒现象;(3)光镜下实验动物肝脏脂肪沉积，肝细胞病理损伤及肝组织炎症反应程度加重，纤维化不明显;(4) ALT，AST水平升高。结论:酒精可以导致肝细胞物质代谢障碍，使肝细胞变性，坏死。 关键词:酒精 灌胃 肝细胞病理损伤 肝细胞变性 Abstract Purpose: it is to study the damage of alcohol to baby white mice’ liver and its possible influence on mechanism. Method: divide 40 baby white mice, which weigh from 18g to 22g, into 4 groups casually. To those mice belonging respectively to the first, second and third group, we pour in turn 10%, 30%, 50% volume of alcohol into their stomachs, once per day. To those belonging to the fourth group, we pour 0.9% volume of physiological saline, once per day lasting 5 weeks. Measure and record the weight of the baby mice once per week, observe and record their mental condition and concurrently measure the content of ALT and AST serum. Five weeks later, kill the bay white mice and take out their livers in the same part of the body to observe the shape. Then we get the results: 1. the weight of baby mice in each group has increased, while those of the first group has increased most and those of the third group has increased least; 2. baby white mice of the first and fourth group have the same appetite as usual and are in good mental health, while those of the second and third group have the phenomenon of being drunk; 3. the experimental animals’ liver fat deposit under the light microscope, pathological damage of liver cells and the response degree of liver tissue’ s inflammation has deepened and the fibrosis is not so clear. 4. The level of ALT and AST has risen. Conclusion: alcohol can cause obstruction to liver cell’s metabolism and denaturalize liver cells as well as make it die. Key words: alcohol; pour in stomach;