氯嘧磺隆降解菌株LW-3的分离及生物学特性研究.pdfVIP

微生物学通报 DEC 20, 2008, 35(12): 1899~1904 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 氯嘧磺隆降解菌株 LW-3 的分离及生物学特性研究 王 哲 孙纪全 马吉平 黄 星 李顺鹏* ( 210095) 摘 要: 从长期受氯嘧磺隆污染的土壤中分离到一株氯嘧磺隆高效降解菌株, 命名为 LW-3, 菌株 LW-3 可以氯嘧磺隆为唯一氮源生长, 接种量为 2% 时, 50 mg/L 氯嘧磺隆经过 7 d, 降解率达 70%~80% 。生理生化实验和 16S rDNA 序列同源性分析, 将菌株 LW-3 归属于假单胞菌属 (Pseudomonas sp.); 菌株的适宜降解条件为; 温度30°C~35°C, pH 6.5~7.2, pH 对菌株LW-3 降解氯 嘧磺隆有明显地影响。 关键词: 氯嘧磺隆, 微生物降解, 假单胞菌属, 生物学特性 Isolation of Strain LW-3 Capable of Degrading Chlorimuron-ethyl and Its Biological Characteristics * WANG Zhe SUN Ji-Quan MA Ji-Ping HUANG Xing LI Shun-Peng (College of Life Sciences, Key Laboratory for Microbiological Engineering of Agricultural Environment, Ministry of Agriculture , Nanjing Agricultural University, Nanjing 210095) Abstract: A bacterium(designated as LW-3), capable of degrading Chlorimuron-ethyl, was isolated from the long-term contaminated soil by Chlorimuron-ethyl. Based on physiological and biochemical analyses and sequences comparison of 16S rDNA, strain LW-3 was identified as Pseudomonas sp. LW-3 could use Chlorimuron-ethyl as sole nitrogen source for growth. The optimum pH and temperature for degradation of Chlorimuron-ethyl were pH 6.5~7.2 and 30°C~35°C, at same temperature the pH change to the Chlorimuron -ethyl degrading influence is large. When the pH and temperature were pH 6.5 and 32°C, 50 mg/L Chlori- muron-ethyl could