Chemical Modification of the Inner and Outer Surfaces of Tobacco Mosaic Virus (TMV) 烟草花叶病毒内外表面的化学修饰.pdfVIP

HHS Public Access Author manuscript A Methods Mol Biol . Author manuscript; available in PMC 2015 August 23. u t h Published in final edited form as: o r Methods Mol Biol . 2014 ; 1108: 173–185. doi:10.1007/978-1-62703-751-8_13. M a n u s c Chemical Modification of the Inner and Outer Surfaces of r i p t Tobacco Mosaic Virus (TMV) Michael A. Bruckman and Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA A Nicole F. Steinmetz u t Department of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, h o Cleveland, OH, 44106, USA r M a Radiology, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106, USA n u s Materials Science and Engineering, Case Western Reserve University, 10900 Euclid Avenue, c r Cleveland, OH, 44106, USA i p t Abstract Viral nanoparticles derive from tobacco mosaic virus (TMV) has broad applications in various fields. We report the purification and chemical engineering of TMV which is a hollow rod-shaped plant viral nanoparticle with modifiable interior and exterior surfaces. Here, we describe methods A to isolate TMV from its tobacco plant host for spatially controlled interior and exterior chemical u t modification and to characterize the resulting TMV hybrid materials. h o r M a Keywords n u s Bioconjugation chemistry; CuAAC (click) chemistry; Tobacco mosaic virus (TMV); Viral c r nanoparticles (VNPs) i p t 1 Introduction Tobacco mosa