非离子表面活性剂对生物丁醇发酵的影响-微生物学报.PDFVIP

784 生 物 工 程 学 报 侯同刚 等/非离子表面活性剂对生物丁醇发酵的影响 Chinese Journal of Biotechnology /cjbcn May 25, 2014, 30(5): 784−792 DOI: 10.13345/j.cjb.140016 ©2014 Chin J Biotech, All rights reserved 组分转化 侯同刚，张跃冬，陈秀芳，关静，牟新东 266101 , , , . . , 2014, 30(5): 784−792. Hou TG, Zhang YD, Chen XF, et al. Effect of non-ionic surfactants on butanol production with Clostridium acetobutylicum ATCC 824. Chin J Biotech, 2014, 30(5): 784−792. 摘 要: 传统的丙酮-丁醇发酵的产物浓度过低 ( 丁醇终浓度约为 1.3 wt%)，导致后期分离成本过高，从而影 响了该过程的经济性，限制了其工业化进程。本文研究了高添加量的小分子非离子表面活性剂对生物丁醇发酵 的影响。以吐温80 为例，实验表明，当表面活性剂添加量超过其临界胶束浓度后，丁醇发酵的终浓度会随着 表面活性剂添加量的增加而增加。当添加量达到 5 wt%时，丁醇终浓度可以达到1.6 wt%，远高于该菌种的抑 制浓度 (0.8 wt%) 。为阐明表面活性剂的作用机理，实验考察了吐温 80 对丁醇的增溶效应以及对发酵菌体表 面亲疏水性的影响。结果表明，吐温80 对丁醇的增溶效果很小，而对菌体表面的亲疏水性有较明显的影响。 : 表面活性剂，吐温 80 ，生物丁醇，毒性，增溶 Effect of non-ionic surfactants on butanol production with Clostridium acetobutylicum ATCC 824 Tonggang Hou, Yuedong Zhang, Xiufang Chen, Jing Guan, and Xindong Mu Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China Abstract: The low butanol concentration of acetone-butanol-ethanol fermentation causes uneconomical product recovery. In this work, the effect of small molecule non-ionic surfactants on butanol fermentation was evaluated, using laboratory stocks of Clostridium acetobutylicum ATCC 824. Non-ionic surfactants substantially increased butanol production when additive amount was higher than 1% ( W/ W). Butanol concentration reached 16.9 g/L with 5% ( W/ W) Twee