两种阳离子纳米基因载体及植物基因介导效果的研究.pdfVIP

生物技术通报 ·研究报告 · B IO TECHN OLO G Y　BULL E T IN 2009年第 6 期 两种阳离子纳米基因载体及植物基因介导效果的研究 宋瑜 　李颖 　崔海信 　李瑶 (中国农业科学院农业环境与可持续发展研究所 ,北京 100081) ( ) ( ) 　　摘 　要 : 　以阳离子聚乙烯亚胺 polyethylen im ine, PE I 和壳聚糖 ch ito san, CS 作为两种植物基因载体 ,分别制备了载基 ( ) ( ) 因 PE I纳米粒 PE I/DNA 和壳聚糖 DNA 纳米粒 CS/DNA ,并对其形态 、粒度分布 、包封率 、DNA 结合的稳定性及纳米颗粒 对 DNA 的保护等方面进行表征 。并以 GFP 基因为报告基因进行植物细胞转染 , 比较两者转化效率 。结果表明 PE I/DNA 纳 米粒稳定性 ,对 DNA 的保护以及转染效率等方面均优于壳聚糖 DNA 纳米粒 。 关键词 : 　聚乙烯亚胺 　壳聚糖 　纳米粒 　基因载体 　转基因 Study on Ca tion ic Nanoparticles a s Gene Carr iers and the Eff ic iency for Tran sferr ing Gene into Plan t Cells Song Yu　L i Ying　Cu i H aixin　L i Yao ( ) CAA S Institu te of E nvironm en t and S usta inable D evelopm en t in A g ricu ltu re, B eij ing 100081 　　Ab s tra c t: 　Polyethyleneim ine ( PE I) and ch ito san (CS) were u sed to p rep are PE IDNA ( PE I/DNA ) and CSDNA ( CS/DNA ) comp lexes a s gene carriers for DNA delivery The characteristic s of the comp lexe s such a s morp hology, p article size, a ssociation effi ciency, stab ility of DNA b inding, and p rotection ab ility to DNA were inve stigatedTran sfection efficiency m ediated by PE I/DNA and CS/DNA were exam ined w ith GFP gene as report gene, re sp ectively The resu lts showed that PE I/DNA nanop article s were sup erior to ch ito sanDNA nanop article s in stab ility, p rotection ab ility of DNA and tran sfection efficiency A rabidops is tha liana to p rotop lasts Key w o rd s: 　Polyethylen im ine　Ch ito