光谱法研究生理条件下药物与蛋白质的相互作用-分析化学专业论文.docxVIP

堇对牛血清白蛋白的荧光有较强的猝灭作用，其荧光猝灭为静态猝灭过程，其作用机 堇对牛血清白蛋白的荧光有较强的猝灭作用，其荧光猝灭为静态猝灭过程，其作用机 制属能量转移机制。从荧光猝灭结果求得不同温度下反应的结合常数K，随反应温度 上升K值下降。由反应焓变、熵变，确定它们间的结合主要是静电力。依据非辐射能 量转移机理，求出了其结合位置和能量转移效率。 关键词：相互作用：蛋白质：药物；荧光光谱；圆二色光谱 Jl Abstract确e Abstract 确e reaction mechanism of small molecules with protein WaS studied by fluorescence spectroscopy(FS)and uV spectroscopy(uv)and circular dichroism(CD)spectrum。Those reactions quenched the fluorescence produced． Chapter one reviews the molecular sDectrum for the reaction of medicine and protein and metal ions and the progress of fluorescence spectroscopy in pharmacentical analysis in recent years；The results and methods for study of reaction mechanism between small molecules with protein，54 documents are quoted． Chapter two：The binding reactions of lomefloxacin-copper(II)complex(LMF-C∞or LMF tO bovine serum albumin(SSA)in physiological solution were investigated by multi．spectroseopy．nle binding constant,the number of binding sites and the binding distsncc between LMFZu 01LMF and BSA were obtained by a fluorescence quenching method and according tO the mechanism of Forster-type dipole-dip}ole non-radioactive energy transfer,respectively．Enthalpy and entropy changes for two systems were calculated to be一7．970 kJ mol～and 47．438 J tool-1 K一1 for UⅥFLBSA,-12．469 kJ reel～and 33．542 J reell K～for LMF-Cu-BSA，respectively．T11e hi曲ly positive values observed for the entropy give evidence for a swong interaction．111e values of△日and AS in twO systerns are similar,indicating that electrostatic interactions in two systems play major role．The effect of LMF—CU or LMF on the conformafion of BSA was alSO analyzed by synchronous fluorescence，three-dimensional fluorescence and circular dichroism spectra．The results showcd that the presence of Cu ion in LMF-Cu can affect the conformation of BSA tO some degree．All theresults revealed that the addition of copper ion promotes the interaction of lomefloxacin with bovine serum albumin． Chapter three：The interaction and the