广西类禽型H1N1猪流感病毒分离鉴定和基因序列的测定分析-预防兽医学专业论文.docxVIP

II II 基化位点。三株分离株和 A／swine／Zhejiang／1／2007 糖基化位点 相同，没有增加也没减少。NA 有 6 个糖基化位点分别为 235(NGSC)、 146(NGTI) 、88(NSSL) 、68（NVSN）、63(NQTY)、8(NNTW)。 G14 、 G21 、 G30 三株流感病 NA 的 毒糖基化位点和 A/swine/Zhejiang/1/2007 代表株基本一致，只是 G21 的第 68 糖基化 位点（NVSN）和代表株的 68 位糖基化位点（NISV）发生了变异。 糖基化位点的增加和减少对分离株的致病力、抗原特性、和药物抗 性影响都比较大。三株分离株 G14、G21、G30 可能在致病力，抗 原特性，和药物抗性上都一定的变化。M 基因对病毒的耐药性起很 大作用，根据药物试验证实，L26F 、V27A 、A30T 、S13N 、G34E 如果这些位点其中一个发生变异将可能直接影响病毒的抗药性。本 实验分离的三株病毒这些位点都没发生变异，这说明这三株病毒对 金刚烷胺类药物不具有抗药性。 关键词：类禽 H1N1 猪流感病毒 分离鉴定 基因克隆 序列分 析 III III ISOLATION AND WHOLE GENOME SEQUENCE ANALYSIS OF AVIAN-LIKE H1N1 INFLUENZA VIRUS IN GUANG XI ABSTRACT Virologic and serological studies were conducted on swine nasal mucus and serum samples collected from Guiping in Guangxi. The results indicated that three of 365 samples, were positive for influenza virus, the isolate rate was 0.82%. All the three isolates were determined as H1N1 subtype influenza virus by hemagglutination-inhibiton and whole genome sequencing, which named G14 、 G21 、 G30 ， respectively. Sequence analysis confirmed that G14、G21、G30 isolates were high related with A/swine/Zhejiang/1/2007, and the amino acid receptors were 108 (Y) 、 148-152(GVTAA) 、 148(W) 、 197(H) 、 204(D) 、 208 (L) 、 209(Y),respectively,which was the same with A/swine/Hungary/19774/2006 and A/swine/Zhejiang/1/2007. Analysis of glycosylation sites found that HA1 had 5 glycosylation sites, HA2 had 2 glycosylation sites,which were 27(NNST) 、 28(NSTD) 、 40(NVTV) 、 212(NHTY) 、 291(NCTT) 、 498(NGTY) 、 557(NGSL), respectively. G14、G21、G30 and A/swine/Zhejiang/1/2007 had the same glycosylation sites, but 21 increased to A/swine/Hungary/19774/2006. Increase or decrease of glycosylation sites lead to the change of pathogenicity、antigenic characteristics and HYPERLINK /dict/search?q=chemoresistanceamp;FORM=BDVSP6 chemoresistance. Analysis NA of glycosylation sites found that NA had 6 glycosylation sites,that were 235(NGSC